| 研究課題/領域番号 |
21K07554
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| 研究機関 | 沖縄科学技術大学院大学 |
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研究代表者 |
TAOUFIQ Zacharie 沖縄科学技術大学院大学, 細胞分子シナプス機能ユニット, スタッフサイエンティスト (10549348)
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| 研究期間 (年度) |
2021-04-01 – 2024-03-31
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| キーワード | iPSC-derived neurons / Synapse / proteomics / psychiatry / Schizophrenia / diagnosis / biomarkers |
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| 研究実績の概要 |
Brain sampling on living psychiatric patient is key but unethical. Here, we have developed a way to perform: a noninvasive/harmless dissection of brain synapses from living psychiatric patients. We combined stem cell reprogramming technology and our powerful UD proteomics approach (Taoufiq et al PNAS 2020). We called this new technology: ‘Personalized Synapse Proteomics’ (PSP). In this year, we validated the concept of a PSP. The primary endpoint was to determine whether we could see alterations of brain synaptic proteins from a living psychiatric patient versus his healthy sibling. 1 we successfully generated iPS cells from blood sample of a local patient suffering from schizophrenia and his healthy sibling. 2 we successfully differentiated of the cells into neurons. 3 we managed to purify enough intact synapses from patient-derived neurons. 4 Using UD proteomics, we could have identified and quantified proteome alterations in brain synapses from a living patient with schizophrenia versus his healthy sibling. These included known schizophrenia disease biomarkers and may encompass dozens of novel and previously invisible ones. Therefore, we concluded that PSP technology prototype worked as predicted. We also filed a patent on a new culture medium recipe that makes psychiatric patients neurons much healthier and more connected.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
1: 当初の計画以上に進展している
理由
We already validated the concept and protocol of PSP.
We have now yet to perform the final step which is to use PSP on all the patient samples.
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| 今後の研究の推進方策 |
We have now performing the final step which is to compare synaptic proteomics of all other patient samples.
We have in total 4 patients for this project,
However we will use PSP on samples received from another funding source.
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| 次年度使用額が生じた理由 |
Reduced activity due to Corona period (shipment delays of products).
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