| 研究課題/領域番号 |
21K12269
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| 研究機関 | 獨協医科大学 |
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研究代表者 |
オオタケサトウ マルセロ 獨協医科大学, 医学部, 助教 (50771896)
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| 研究分担者 |
迫 康仁 旭川医科大学, 医学部, 教授 (40312459)
サトウ 恵 新潟大学, 医歯学系, 准教授 (70601813)
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| 研究期間 (年度) |
2021-04-01 – 2024-03-31
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| キーワード | Ecohealth / One-health / environmental DNA / STH / qPCR |
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| 研究実績の概要 |
Goal: Improvement of soil transmitted helminths-specific DNA detection method.
The analyses of DNA sequences of the parasite species causing STH retrieved from the database allowed us to develop suitable probes for STH detection.
We designed the environmental DNA detection assay for soil samples, using mitochondrial COX1 gene. The specific sets of primers and probes were designed within an expected short length of the amplified DNA (about 100 to 200 bp).
Tests using DNA extracted from adult worms (target and non-target) showed the assays are suitable with a high specificity. However, it needs improvement on the sensitivity, for detection of small quantities of target DNA usually found eDNA.
We are now working on a new technique based in multi-probe detection which increases the sensitivity of the assay without loss in specificity (as reported by our group in detection of Opisthorchis viverrini in eDNA from water samples).
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
3: やや遅れている
理由
The project is progressing smoothly at the laboratory, however we still are experiencing delay in field samples.
Regarding the developed assays, we think we can increase the sensitivity of the technique using a multi-probe system, and we are on the way to standardize and test the assay using soil samples.
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| 今後の研究の推進方策 |
We are looking forward to standardize the multi-probe detection assay and apply it to eDNA extracted from soil samples from the field.
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| 次年度使用額が生じた理由 |
The reason for the incurring amount to be used next fiscal year is basically the travel restrictions occurred due the COVID19 pandemics.
We hope to use the expected amount for the postponed field surveys, improvement of laboratory assays, attend international meetings, and use it for cover publication fees.
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