研究実績の概要 |
The STH to be detected were A lumbricoides, T trichiura and Hookworms (A duodenale, and N americanus). From the aligned sequences of cox1 gene by species of parasite we determine specific variable/conserved areas and design sets of primers/probes to be used in multiplex qPCR. The assay was screened in silico for specificity. Worms' DNA were used to attest the usefulness of the system. Then, soil samples from endemic area were used. The soil eDNA was extracted and tested, completing the goals of this research project with the correlation of positive STH cases with the environmental contamination. Furthermore, we are still analyzing more soil samples to increase the number of tests, and use it to design an accurate risk mapping of STH infection sites from endemic areas. Paper in preparation
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