| 研究実績の概要 |
The key objective of the project was to make clear the possibility of utilizing waste-derived DNA for the preparation of functional materials using the thermal processing method. The HT synthesis of DNA biodots using a conventional HT reactor and a microwave reactor under the same conditions was compared and it was shown that biodots prepared using the microwave reactor had 1.5-2-fold higher fluorescence.
The process of nucleic acids thermal decomposition by hydrothermal and pyrolytic methods was systematically investigated. The synthetic methods for preparation of fluorescent nanoparticles (biodots) from nucleic acids, DNA and RNA were elaborated and the possibility to control the fluorescent intensity and fluorescence color of product was demonstrated. The mechanism of nucleic acid biodots formation was shown to include degradation of ribose sugar, depurination, depyrimidation and consequent fusion of heteroaromatic nucleobases. The structure of biodots was clarified.
The application of nucleic acids for analytical detection and Hg2+, Ag+, and Cu2+ ions at ppm concentrations as well as bioimaging was demonstrated. In contrast, the application of DNA thermal decomposition products such as biochars for adsorption and catalytic applications was shown to be limited.
The conducted research provided important knowledge on the scope and limitations of DNA biomass-derived products prepared by thermal processing and represents a valuable technology in addition to the existing sustainable biomass processing methods.
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