| 研究課題/領域番号 |
21K12665
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| 研究機関 | 広島大学 |
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研究代表者 |
カサラゴッド デイーパカマス 広島大学, 医系科学研究科(医), 助教 (40773908)
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| 研究期間 (年度) |
2021-04-01 – 2024-03-31
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| キーワード | optical imaging / fluorescence microscopy / 3d brain imaging / neuroinformatics |
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| 研究実績の概要 |
The purpose of the proposed research was to establish deep ultraviolet fluorescence microscope for 3-dimensional (3D) brain imaging specifically towards mesoscopic quantification of the anatomical structures of interest. Secondary goal included developing open microscopy and open data toolkit to be made available in the public domain.
In FY2021, a prototypical fluorescence microscope for automated serial slicing and high resolution widefield block-face imaging across the whole brain or tissue block of interest was successfully implemented. Open source based image acquisition and analysis pipeline along with a graphical user interface for easy use was also implemented. Consolidating the engineering aspects of the work for submitting a manuscript to a technical journal is ongoing.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
The first phase (FY2021) of the development of fluorescence microscope for whole brain imaging was successfully implemented. This included extending the fluorescence microscope towards automated serial block-face imaging of the whole brain. Image acquisition and analysis pipeline was developed using custom developed open source based software using Python as the programming language as well as integrating the existing open source tools, as required. A graphical user interface was also developed for ease of use. Consolidating the engineering aspect to be submitted to a technical journal is under consideration. The current status of the project includes work towards development of stereology tools for proper 3d quantification from serial sections imaging carried out by this microscope.
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| 今後の研究の推進方策 |
The plans for the phase2 (FY2022) and phase3 (FY2023) of the 3-dimensional (3D) fluorescence microscopy for brain imaging are as follows:
FY2022 research project would include development of stereological protocols for 3D volumetric analysis of the brain structures, towards elucidating the changes in the brain anatomical structures with respect to normal and diseased mouse models.
In FY2023, the chief focus would be consolidate all 3D microscopy based tools developed using deep ultraviolet microscope to be put into the open domain as the ‘Do-It-Yourself’ 3D microscope for micro-macro analysis so as to enable smaller neuroscience labs with minimal resources to set-up their own version of 3D microscope for a community based approach towards elucidating brain connectivity.
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| 次年度使用額が生じた理由 |
A certain amount of the budget in the last financial year (FY2021) was kept for the end of financial year expenses, as a contingency plan. The whole amount was not utilized and so the balance amount is incurred to the next fiscal year (FY2022). This amount along with the budget of FY2022 would be utilized for purchasing mice for the imaging studies using animal disease models.
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