| 研究課題/領域番号 |
21K12665
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| 研究機関 | 広島大学 |
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研究代表者 |
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| 研究期間 (年度) |
2021-04-01 – 2024-03-31
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| キーワード | optical imaging / 3d brain imaging / fluorescence microscopy / neuroinformatics |
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| 研究実績の概要 |
The purpose of the proposed research was to develop open source microscopy tools for 3D brain imaging using novel light source for fluorescence microscopy in the deep ultraviolet spectrum.
In FY2021, a prototypical fluorescence microscope for automated serial slicing and high resolution widefield block-face imaging across the whole brain or tissue block of interest was successfully developed. A manuscript submitted on the technical aspects is currently in second phase of revision.
In FY2022, the applications for quantitative analysis was carried out on normal mouse brains. The study is running smoothly as expected.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
In FY2022, tools for quantitative analysis of the cytoarchitecture of the mouse brains was developed using the microscope developed. A technical paper is under final phase of revision to be considered for publication. Further applications of functional imaging are under consideration.
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| 今後の研究の推進方策 |
The plans for the phase3 (FY2023) of the 3-dimensional (3D) fluorescence microscopy for brain imaging are as follows:
In FY2023, the chief focus would be consolidate all 3D microscopy based tools developed using deep ultraviolet microscope to be put into the open domain as the ‘Do-It-Yourself’ 3D microscope for micro-macro analysis so as to enable smaller neuroscience labs with minimal resources to set-up their own version of 3D microscope for a community based approach towards elucidating brain connectivity.
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| 次年度使用額が生じた理由 |
In the last financial year (FY2022), budget reserved for conferences were not utilized as expected to due to the still ongoing COVID pandemic situation. Moreover, these funds were reserved towards covering the publication costs which is expected in FY2023.
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