| 研究実績の概要 |
In the last year, the extraction and purification procedures of seaweed phytosterols were optimized. So, in this year, the quantitation method was developed and validated. Then, we applied the established methods to 16 batches of three commonly consumed brown seaweeds, including 4 batches of Hijiki, 4 batches of Wakame, and 8 batches of Kombu.
Next, the anti-AD potential of seaweed-derived phytosterols were assessed. Cell viability significantly decreased to 66.6% ± 19.7% in the PA-treated group compared to the control group. However, it significantly increased to 95.4% ± 10.8% in the 10 ug/mL phytosterol-treated group and 110.9% ± 11.4% in the 30 ug/mL phytosterol-treated group compared to the PA-treated group. Thus, the cytoprotective effects of phytosterol-treated phytosterols were suggested. The total TG content significantly increased to 11.6 ± 5.3 nmol in the PA-treated group compared to 1.7 ± 0.3 nmol in the control group. However, it significantly decreased to 9.4 ± 4.7 nmol in the phytosterol-treated group compared to the PA-treated group. A similar trend was observed for various fatty acyl chains in the TG fatty acid composition assessed by LC-MS/MS. These results suggest that seaweed-derived phytosterols inhibit TG accumulation.
To summary, in this study, the phytosterols from seaweed were extracted and purified using an optimized procedure. The phytosterols were profiled and determined among different seaweeds. Moreover, the beneficial effects of seaweed-derived phytosterols on AD-modelled PC12 cells were identified.
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