| 研究課題/領域番号 |
21K14812
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| 研究機関 | 北海道大学 |
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研究代表者 |
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| 研究期間 (年度) |
2021-04-01 – 2025-03-31
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| キーワード | Hijiki / obesity / Sphingolipid metabolism / LC/MS |
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| 研究実績の概要 |
The project is going well, we have identified the active fraction and component of hijiki responsible for SMS inhibition. The in vitro assays were performed using the active fraction of hijiki in C3A cells to reveal its action against lipid droplet accumulation. We have found that hijiki active fraction significantly suppressed the lipid droplet accumulation. However, these are preliminary results, we are examining in detail by various analysis including LC/MS analysis of lipid contents changed upon hijiki active fraction treatment. Further, the active fraction is purified by column chromatography after repetitive purification, HRMS and NMR data of the pure compound were obtained. The structural analysis is in progress after gathering all the data.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
Currently, the project is running smoothly, and we are at the stage to conduct the in vitro experiments this year using the purified active compound of hijiki. The reasons for choosing the above option is due to challenges associated with the compound purification as well as establishment of LC/MS based SMS inhibition assay.
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| 今後の研究の推進方策 |
In the future, I aim to conduct the experiments after purifying the large amount of Hijiki derived active compound responsible for SMS inhibition. Hence, in this year the research will be focused on large scale purification of Hijiki derived active compound and confirming SMS inhibition by both HPLC with fluorescent detection and LC/MS based assays. Then in vitro experiments using isolated compound and C3A cells stimulated with lipid droplets will be planned as proposed in the plan. Furthermore, experiments including action of the active component on lipid droplet oxidation will be performed.
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| 次年度使用額が生じた理由 |
The amount was unable to use for the consumables as it is very low.
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