| 研究実績の概要 |
Isolation, purification, and quantification:
-Momilactones A (MA) and B (MB) were completely isolated and purified from rice husks, of which, 100 mg MA and 200 mg MB from 20 kg rice husk. On the other hand, we completely isolated p-coumaric acid and tricin from rice husk. The purification is in progress. In addition, we preliminarily quantified MA, MB, p-coumaric acid and tricin in some Japanese rice varieties (brown rice). A new method for optimizing the detection of momilactones and other active compounds was developed.
Screening of anticancer activity (cytotoxicity):
-We completely investigated the cytotoxicity of MA and MB on blood cancer cell lines including Meg-01, K562, HL60, Mino, U266, KMS-11 by MTT assay. We completely achieved IC50 value for the cytotoxicity of MA and MB on both tested normal cell and cancer cell lines.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
- We had a good preparation for extracting and isolating target compounds from rice husks, therefore, I could get the desired amount of momilactones and relevant compounds;
- We had a good collaboration with Hematology Lab led by Prof. Dr. Takami Akiyoshi at Aichi Medical University, therefore, the screening cytotoxicity of momilactones on blood cancer cell lines could be done successfully;
- We have not carried out cytotoxicity on lung and gastric cancer cell lines because of the delay in ordering cell lines and chemicals caused by COVID-19.
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| 今後の研究の推進方策 |
- To continue isolating and purifying bioactive compounds and screening the cytotoxicity on other cancer cell lines;
- To determine anticancer mechanism of isolated compounds, especially, momilactones A and B;
- To evaluate the effect of in vitro digestion on accessibility, availability, and activity of momilactones A and B.
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