研究課題/領域番号 |
21K15094
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研究機関 | 沖縄科学技術大学院大学 |
研究代表者 |
HUSNIK Filip 沖縄科学技術大学院大学, 進化・細胞・共生の生物学ユニット, 准教授 (30886130)
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研究期間 (年度) |
2021-04-01 – 2023-03-31
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キーワード | proteomics / metabolomics / symbiosis / bacteria / insects / immunohistochemistry / 3D imaging / protein import |
研究実績の概要 |
Metabolomics and proteomics protocols were established for the model system. All tissues of interest were prepared, their composition analyzed with mass spectrometry, and numerous metabolites identified. Volume imaging(microCT, FIB-SEM, confocal z-stacks) was used to understand host-symbiont integration. Protein candidates for future antibody labeling were identified.
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現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
Proteomics and metabolomics progressing more smoothly than expected. More imaging methods than originally planned were established, but antibody labeling has not been carried out yet.
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今後の研究の推進方策 |
I will build on proteomics and metabolomics experiments that were already successfully finished but focus on improving subcellular and spatial resolution. Protein localization with antibodies is to be carried out.
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次年度使用額が生じた理由 |
Expenses for metabolomics and antibody generation have to be spent in batches (multiple samples at once), so exact planning is not possible. No travel due to travel restrictions.
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