| 研究課題/領域番号 |
21K15214
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| 研究機関 | 国立研究開発法人理化学研究所 |
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研究代表者 |
Sekulic Vladislav 国立研究開発法人理化学研究所, 脳神経科学研究センター, 基礎科学特別研究員 (60865492)
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| 研究期間 (年度) |
2021-04-01 – 2024-03-31
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| キーワード | calcium imaging / hippocampus / memory encoding |
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| 研究実績の概要 |
In FY2021, I successfully implanted GRIN lenses and performed 1-photon calcium imaging recordings from 15 mice constituting the entire dataset for a major experiment.
I acquired more 1-photon miniature endoscope hardware and a filter set for imaging from cells with red fluorescence to continue the experiment combining in vivo activity recording and immediate early gene (IEG) memory labelling in the same mice. These recordings will be performed in FY2022.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
1: 当初の計画以上に進展している
理由
I had initially anticipated that the main recordings for the calcium imaging dataset would take the entire FY year or even 2 years, but I was able to complete them within 6 months. This will allow further recordings and expanded research results. The benefit of the Miniscope 1-photon system that I acquired using KAKENHI funds is tremendous as it is a reliable and flexible system that performed much more smoothly than anticipated. Furthermore, as I had gotten comfortable and highly capable with the GRIN lens surgeries, all of my mice had successful implants and could be recorded from (success rate nearly 100%), allowing for rapid acquisition of a large dataset.
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| 今後の研究の推進方策 |
The further steps to be taken in FY2022 is to image from a new set of mice using simultaneous red imaging of memory-dependent molecular markers that label novel engram forming cells, while also imaging dynamic in vivo activity using the previous 1-photon Miniscope. This will require the use of two scopes, one of which I will assemble from parts I already acquired using my KAKENHI funds.
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| 次年度使用額が生じた理由 |
I plan on acquiring more GRIN lenses and 1-photon Miniscope hardware for performing additional experiments. Additionally, I will purchase a new Cre genotype mouse line for these more refined experiments.
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