| 研究実績の概要 |
To understand how TFE3/B contribute to adaptive responses leading to generation of dormant and quiescence cancer cells, I applied a combination of molecular techniques including siRNA, shRNA and CRISPR/Cas9 KO of TFE3/B in a number of PDAC cell lines with very different phenotype and performed metabolic, proliferation studies and sensitivity to current standard chemotherapies. Through a combination of RNA-seq and ChIP-seq I have profiled TFE3/B contribution to microenvironmental stress over time.
Using OSCAR reporter, I demonstrated phenotypic heterogeneity and the presence of transcription quiescence cells (a hallmark of dormancy) even among isogenic population (Cell lines) and demonstrated that, at least in vitro, standard chemotherapy leads to an increased in this dormant population.
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