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A HER2 targeting bi-modal MRI/NIR-II micelles with a diameter about 30 nm has been developed. Antibodies were bound to the probe through their amine groups on carboxyl-functionalized micelles via EDC/NHS chemistry. The ratios of coupling carboxyl groups on accessibility of immobilized anti-bodies and detection limits of specific antibody-antigen affinity were examined. Bimodal imaging probes with different coupling ratios of antibodies on their surface were investigated to determine the optimal surface density. Assays of target-binding abilities of the Herceptin-bound probes were obtained when various concentrations of micelles were applied for specific detection of antigens. Targeting ability was verified with fluorescence imag-es of SKBR-3 and MCF-7 cells incubated with these probes.
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