| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
Large bone defects that cannot heal spontaneously are increasing in association with an ageing society in the inability to adequately treat these defects represents an unmet medical need. This study aims to show that a complex of osteogenic cells, such as immature osteoblast-like cells and a poly-lactic acid polymer based on three-dimensional biodegradable scaffolds, has the potential to overcome this drawback. The main aim of our study is to show that the autogenic transplantation of immature osteoblast-like cells and a 3DPLA nanoarchitecture scaffold represents a viable new bone tissue engineering methodology for large jawbone defects.
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| 今後の研究の推進方策 |
To verify the jawbone regeneration ability of the MCOB-3DPLA constructs, Micro CT analysis, Histological (HE and Masons trichome staining) and immunobiological (CD31 and Osteocalcin) analysis will be performed. Micromechanical properties of the regenerated bone in the mouse maxilla defect model using nanoindentation measurements will be carried out, an effective technique for assessing the hardness of bone tissue. The nano indented measurements will be taken from the centre of the defect area in the MCOB-3DPLA, cytrans, 3DPLA and empty defect groups. The osseointegration ability of the regenerated bone will be evaluated by placing a dental implant at eight weeks post-transplantation to assess the tolerance to implant therapy. The implants will be placed in the area of regenerated bone (M1 area) in all of the experimental groups, and osseointegration will be tested after four weeks by micro CT and histological analysis.
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