| 研究課題/領域番号 |
21K16962
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| 研究機関 | 東北大学 |
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研究代表者 |
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| 研究期間 (年度) |
2021-04-01 – 2024-03-31
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| キーワード | Regenerated Bone / Nanoindentation / Implant Therapy / Functional Bone / Micro-CT analysis |
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| 研究実績の概要 |
The jawbones from the mice alveolar bone defect models were examined by Micro-CT scanning and histological analysis to assess new bone formation in the defect area at 4 and 8 weeks after implantation. The results of 3D image analysis and Hematoxylin and Eosin staining demonstrated that implanted MCOB-3DPL constructs have successfully regenerated bone within bone defects and is significantly higher than the other groups at 8 weeks post-implantation. Nanoindentation analysis has shown that regenerated bone induced by MCOB-3DPL constructs has micromechanical properties comparable to the native bone.
The results of implant therapy by Micro-CT and histological analysis revealed that regenerated bone formed by MCOB-3DPL constructs possesses osseointegration ability.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
It has been shown previously that this type of growth can be achieved through a three-dimensional arrangement of osteogenic cells, such as immature osteoblasts, in a scaffold. In our present study, we describe the development of a three-dimensional cotton-like scaffold structure produced by novel electrospinning technology using 3DPL. This 3DPL scaffold complex is suitable for MCOB differentiation to regenerate large jawbone defects. Most of the results are as expected and favourable towards the MCOB-3DPLA group. However, in one of the control group, we used cytrans is also showing comparable results to MCOB-3DPLA group. So are trying to find out are any significant differences between the measuring parameters of new bone and functional property between these two groups.
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| 今後の研究の推進方策 |
Immunohistochemical analysis of the defect area for selected bone markers to evaluate active remodelling processes around the regenerated new bone will be investigated.
Histological and 3D imaging analysis will be performed after four weeks of implantation to evaluate the levels of osseointegration, and the implant survival rates.
Scaffolds designed for tissue engineering need to be biocompatible with the regenerating cells and provide a suitable environment for attachment, proliferation and differentiation. Viable cells within the scaffold are required for bone regeneration, whereby cells secrete extracellular matrix or growth factors to induce new bone formation. Therefore, a Live dead assay will be performed to assess the viability of MCOBs on the seeded 3DPL4 scaffolds.
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