| 研究課題/領域番号 |
21K18048
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| 研究機関 | 国立研究開発法人理化学研究所 |
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研究代表者 |
Koh IsabelSiewYin 国立研究開発法人理化学研究所, 開拓研究本部, 特別研究員 (90868415)
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| 研究期間 (年度) |
2021-04-01 – 2023-03-31
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| キーワード | Cube device / Multi-directional / Vascularisation |
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| 研究実績の概要 |
The objective was to create a precisely-positioned blood vascularisation channel in a modular cube culture device. 3D printing of carbohydrate glass was used to create a water-dissolvable helix-shaped mould that was then placed in hydrogel in the cube. Once the gel was cured, the mould was removed by immersing in phosphate buffer solution (PBS), leaving behind a mould-shaped pocket in the gel. Endothelial cells were then seeded in the helix-shaped channels that remained in the gel to create a vascular channel. Imaging of the vascular channel was achieved by taking images from multiple directions of the cube and combining the images together. This achievement demonstrated the advantage of the cube device in precisely controlling the positioning of cells in 3D culture, and multi-directional imaging. The development of this protocol opens up possibilities to generate complex organoids in the cube device.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
1: 当初の計画以上に進展している
理由
The achievement to control the positioning of cells in the cube, and establishment of method to precise create a channel in the cube by punching is currently being applied to other organs to create organoids with more complex structure and a mix of different cell types.
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| 今後の研究の推進方策 |
With the current method developed, the channel sizes are approximately 1 mm in diameter, which is too large for most applications. In the future, other methods for creating smaller diameter channels are being explored.
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| 次年度使用額が生じた理由 |
Amount too small to buy anything useful
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