| 研究実績の概要 |
Several oral symptoms and diseases of unknown etiology such as oral burning sensation, taste disturbance and lichen planus are most commonly prevalent in post-menopausal females. Although there are no identifiable causes, psychological stress and low estrogen level may be attributed etiologically to these symptoms and diseases. However, the exact effects of psychological stress and estrogen deficiency on the oral symptoms in the post-menopausal females have not been demonstrated. The proteomic analysis of salivary proteins, oral microbiome analysis and genome-wide analysis of oral mucosa in the postmenopausal state may help to understand the etiology of the symptoms and diseases. Therefore, I will perform proteomic, metagenomic and genome wide analyses of the samples obtained from oral cavity in the ovariectomized and chronic stress rat model.
For the first phase of experiment, I performed RNA-seq analysis of submandibular glands between the psychologically stressed rats and control. I identified some candidate genes that are altered in the submandibular gland of psychologically stressed rats. I am validating those genes using qRT-PCR and immunofluorescence. After, validating those genes, the result will be able to identify the genes most affected by psychological stress in submandibular gland. The result might provide a hint on how psychological stress affects oral diseases.
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| 今後の研究の推進方策 |
For the second year, I have divided the rats into six groups as proposed. The rats were housed for three months are now undergoing the psychological stress protocol. After, a month of stress protocol the samples collected from the rats will be subjected for proteomic, genetic and metagenomic analyses.
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| 次年度使用額が生じた理由 |
In the first year, I used the research grant for the initial phase of experiment. The experiment went smoothly as planned. Therefore, a small amount of grant is remaining. The remaining grant from the first year will be used in the second year to perform the proteomic, genetic and metagenomic analyses as planned.
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