ナノ制限空間における界面動電現象の極限可視化技術

2013 年度 研究成果報告書

• 研究課題/領域番号: 22686021
• 研究種目: 若手研究(A)
• 配分区分: 補助金
• 研究分野: 流体工学
• 研究機関: 京都大学 (2012-2013); 大阪大学 (2010-2011)
• 研究代表者: 新宅 博文 京都大学, 工学(系)研究科(研究院), 助教 (80448050)
• 研究期間 (年度): 2010-04-01 – 2014-03-31
• キーワード: 界面動電現象 / ナノ制限空間 / 生体高分子 / 1分子計測 / MEMS流体

研究概要

本研究では，ナノ制限空間におけるイオン流動および界面動電現象を対象とした可視化計測技術を開発し，詳細流動を明らかにすると共に，物理現象を利導した新しい単一分子分析技術の基盤を構築することを目的とした．具体的には，まず，ナノ流路およびナノポアにおける生体高分子の流動に関して可視化流動計測を行い，ナノスケール構造の拘束が生体高分子の流動性を低下させる事を明らかにした．次に，計測技術を拡張し，微小ポアを用いた細胞電気穿孔法におけるイオン・生体高分子流動の計測を行い，局所・瞬時電場の推定を可能とした．最後に細胞電気穿孔法と分子ふるいの効果を利用したRNAおよびDNA同時抽出・分離法に関して検討した．

研究成果

• [雑誌論文] On-chip Separation and Analysis of RNA and DNA from Single Cells (2014)
  • 著者名/発表者名: Hirofumi Shintaku, Hidekazu Nishikii, Lewis A. Marshall, Hidetoshi Kotera, and Juan G. Santiago
  • 雑誌名: Analytical Chemistry 巻: Vol.86, No.4 ページ: 1953-1957
  • 査読あり
• [雑誌論文] Jun Miyake, and Satoyuki Kawano, Measurement of Local Electric Field in Microdevices for Low-Voltage Electroporation of Adherent Cells (2014)
  • 著者名/発表者名: Hirofumi Shintaku, Kazumi Hakamada, Hiroshi Fujimoto, Takeshi Nagata, Jun Miyake, and Satoyuki Kawano
  • 雑誌名: Microsystem Technologies 巻: Vol.20, Issue 2 ページ: 303-313
  • 査読あり
• [雑誌論文] Wide-Range Frequency Selectivity in Acoustic Sensor Fabricated Using Microbeam Array with Non-uniform Thickness (2013)
  • 著者名/発表者名: Hirofumi Shintaku, Takayuki Kobayashi, Kazuki Zusho, Hidetoshi Kotera, and Satoyuki Kawano
  • 雑誌名: Journal of Miromechanics and Microengineering 巻: No.23 ページ: (115014-1)-(115014-7)
  • 査読あり
• [雑誌論文] Electrically Evoked Auditory Brainstem Response by Using Bionic Auditory Membrane in Guinea Pigs (2013)
  • 著者名/発表者名: Hirofumi Shintaku, Takatoshi Inaoka, Takayuki Nakagawa, Satoyuki Kawano, and Juichi Ito
  • 雑誌名: Journal of Biomechanical Science and Engineering 巻: Vol.8, No.3 ページ: 198-208
  • 査読あり
• [雑誌論文] Development of Microfabricated Device for Low-Voltage Electroporation of Adherent Cells (2013)
  • 著者名/発表者名: Kazumi Hakamada, Hirofumi Shintaku, Takeshi Nagata, Hiroshi Fujimoto, Satoyuki Kawano, and Jun Miyake
  • 雑誌名: Journal of Bioscience and Bioengineering 巻: Vol.115, Issue3 ページ: 314-319
  • 査読あり
• [雑誌論文] Tracking Single-Particle Dynamics via Combined Optical and Electrical Sensing (2013)
  • 著者名/発表者名: Naoya Yukimoto, Makusu Tsutsui, Yuhui He, Hirofumi Shintaku, Syoji Tanaka, Satoyuki Kawano, Tomoji Kawai, and Masateru Taniguchi
  • 雑誌名: Scientific Reports 巻: Vol.3 ページ: (1855-1)-(1855-7)
  • 査読あり
• [雑誌論文] Development of Micro-Vibrating Flow Pumps Using MEMS Technologies (2012)
  • 著者名/発表者名: Osman Omran Osman, Hrofumi Shintaku, and Satoyuki Kawano
  • 雑誌名: Microfluidics and Nanofluidics 巻: Vol.13, Issue 5 ページ: 703-313
  • 査読あり
• [雑誌論文] Electrokinetic Flow Dynamics of Weakly Aggregated λDNA Confined in Nanochannels (2011)
  • 著者名/発表者名: Satoshi Uehara, Hirofumi Shintaku, and Satoyuki Kawano
  • 雑誌名: Journal of Fluids Engineering, Transaction of the ASME 巻: Vol.133 ページ: (121203-1)-(121203-8)
  • 査読あり
• [雑誌論文] Piezoelectric Materials Mimic the Function of the Cochlear Sensory Epithelium (2011)
  • 著者名/発表者名: Takatoshi Inaoka, Hirofumi Shintaku, Takayuki Nakagawa, Satoyuki Kawano, Hideaki Ogita, Tatsunori Sakamoto, Shinji Hamanishi, Hiroshi Wada, and Juichi Ito
  • 雑誌名: Proceedings of the National Academy of Sciences of the United States of America 巻: Vol.108, No.45 ページ: 18390-18395
  • 査読あり
• [雑誌論文] Oxygenation to Bovine Blood in Artificial Heart and Lung Using Vibrating Flow Pump : Experiment and Numerical Analysis Based on Non-Newtonian Model (2010)
  • 著者名/発表者名: Hirofumi Shintaku, Tsubasa Yonemura, Kazuaki Tsuru, Takashi Isoyama, Tomoyuki Yambe, and Satoyuki Kawano
  • 雑誌名: Journal of Fluid Science and Technology 巻: Vol.5, No.2 ページ: 292-304
  • 査読あり
• [雑誌論文] Culturing Neurons on MEMS Fabricated P(VDF-TrFE) Films for Implantable Artificial Cochlea (2010)
  • 著者名/発表者名: Hirofumi Shintaku, Takashi Tateno, Nobuyoshi Tsuchioka, Harto Tanujaya, Takayuki Nakagawa, Juichi Ito, and Satoyuki Kawano
  • 雑誌名: Journal of Biomechanical Science and Engineering 巻: Vol.5, No.3 ページ: 229-235
  • 査読あり
• [雑誌論文] Development of Piezoelectric Acoustic Sensor with Frequency Selectivity for Artificial Cochlea (2010)
  • 著者名/発表者名: Hirofumi Shintaku, Takayuki Nakagawa, Dai Kitagawa, Harto Tanujaya, Satoyuki Kawano, and Juichi Ito
  • 雑誌名: Sensors and Actuators A : Physical 巻: Vol.158, Issue 2 ページ: 183-192
  • 査読あり
• [雑誌論文] Developments of Coarse Graining DNA Models for Single Nucleotide Resolution Analysis (2010)
  • 著者名/発表者名: Kentaro Doi, Tomoaki Haga, Hirofumi Shintaku, and Satoyuki Kawano
  • 雑誌名: Philosophical Transactions of the Royal Society A 巻: Vol.368 ページ: 2615-2628
  • 査読あり
• [学会発表] Development of Acoustic Resonator with Non-Uniform Thickness and Mechanical Property for Wide Frequency Range (2012)
  • 著者名/発表者名: Takayuki Kobayashi, Kazuki Zusho, Hirofumi Shintaku, and Satoyuki Kawano
  • 学会等名: 2012 ASME-ISPS /JSME-IIP Joint International Conference on Micromechatronics for Information and Precision Equipment MIPE2012
  • 発表場所: Santa Clara, California, USA
  • 年月日: 2012-07-19
• [学会発表] Theoretical Prediction of Ionic Current Induced by λDNA Flow Through Nano Gap Electrodes (2012)
  • 著者名/発表者名: Satoshi Uehara, Makusu Tsutsui, Hirofumi Shintaku, Kentaro Doi, Masateru Taniguchi, Satoyuki Kawano, and Tomoji Kawai
  • 学会等名: International Symposium on Innovative Nanodevices ISIN 2012
  • 発表場所: Nagoya, Japan
  • 年月日: 2012-03-21
• [学会発表] Improvement of Pumping Performance of micro-Vibrating Flow Pumps by Controlling Valve Motion (2012)
  • 著者名/発表者名: Osman Omran Osman, Motonori Hirata, Hirofumi Shintaku, and Satoyuki Kawano
  • 学会等名: International Symposium on Innovative Nanodevices ISIN 2012
  • 発表場所: Nagoya, Japan
  • 年月日: 2012-03-21
• [学会発表] Optical and Electrical Measurement of Flow Field in Nano Confined Space : Translocation of λDNA through Electrode Embedded In-Plane Nanopore Device (2012)
  • 著者名/発表者名: Hirofumi Shintaku, Makusu Tsutsui, Masateru Taniguchi, Tomoji Kawai, and Satoyuki Kawano
  • 学会等名: International Symposium on Innovative Nanodevices ISIN 2012
  • 発表場所: Nagoya, Japan
  • 年月日: 2012-03-21
• [学会発表] Theoretical Models for Moving λDNA in Nanogap Electrodes (2011)
  • 著者名/発表者名: Satoshi Uehara, Makusu Tsutsui, Hirofumi Shintaku, Msateru Taniguchi, Satoyuki Kawano, and Tomoji Kawai
  • 学会等名: International Workshop on Micro/Nano-Engineering
  • 発表場所: Kyoto, Japan
  • 年月日: 2011-12-18
• [学会発表] Experimental System for Performance Evaluation of Micro-Vibrating Flow Pumps (2011)
  • 著者名/発表者名: Osman Omran Osman, Hirofumi Shintaku, and Satoyuki Kawano
  • 学会等名: International Workshop on Micro/Nano-Engineering
  • 発表場所: Kyoto, Japan
  • 年月日: 2011-12-18
• [学会発表] Control of DNA Conformation Using Rapid Change of Applied Voltage to Interdigitated Electrodes (2011)
  • 著者名/発表者名: Naoya Yukimoto, Satoshi Uehara, Hirofumi Shintaku, and Satoyuki Kawano
  • 学会等名: International Workshop on Micro/Nano-Engineering
  • 発表場所: Kyoto, Japan
  • 年月日: 2011-12-18
• [学会発表] Development of Non-uniform Thick Micro Beam Array for Acoustic Resonators with Wide Frequency Range (2011)
  • 著者名/発表者名: Takayuki Kobayashi, Hirofumi Shintaku and Satoyuki Kawano
  • 学会等名: International Workshop on Micro/Nano-Engineering
  • 発表場所: Kyoto, Japan
  • 年月日: 2011-12-17
• [学会発表] Experimental Study on Flow Control of LambdaDNA in Electrode-Embedded Microchannel (2011)
  • 著者名/発表者名: Satoshi Uehara, Naoya Yukimoto, Hirofumi Shintaku, and Satoyuki Kawano
  • 学会等名: Proceedings of ASME-JSME-KSME Joint Fluids Engineering Conference 2011
  • 発表場所: Hamamatsu, Japan
  • 年月日: 2011-07-26
• [学会発表] Development of Bionic Auditory Membrane with Non-Uniform Thickness for Acoustic Sensor with Wide-Range Frequency Selectivity (2011)
  • 著者名/発表者名: Hirofumi Shintaku and Satoyuki Kawano
  • 学会等名: Proceedings of ASME-JSME-KSME Joint Fluids Engineering Conference 2011
  • 発表場所: Hamamatsu, Japan
  • 年月日: 2011-07-26
• [学会発表] Flow Visualization around Actuating Valve of Micro-Vibrating Flow Pump (2011)
  • 著者名/発表者名: Osman Omran Osman, Hirofumi Shintaku, and Satoyuki Kawano
  • 学会等名: Proceedings of ASME-JSME-KSME Joint Fluids Engineering Conference 2011
  • 発表場所: Hamamatsu, Japan
  • 年月日: 2011-07-25
• [学会発表] Development of Bionic Auditory Membrane for Implantation into Cochleae of Guinea Pigs (2010)
  • 著者名/発表者名: Toshiya Kanbe, Hirofumi Shintaku, Satoyuki Kawano, Takayuki Nakagawa, and Juichi Ito
  • 学会等名: Proceedings of Sixth International Symposium on Meniere's Disease and Inner Ear Disorders
  • 発表場所: Kyoto, Japan
  • 年月日: 2010-11-26
• [学会発表] Recording Evoked Activity of Neocortical Neurons on Multi-Electrode-Array Substrates in Response to Output Signals from Bionic Auditory Membrane (2010)
  • 著者名/発表者名: Yobuyosi Tsuchioka, Hirofumi Shintaku, Takashi Tateno, Satoyuki Kawano, Takayuki Nakagawa, and Juichi Ito
  • 学会等名: Proceedings of Sixth International Symposium on Meniere's Disease and Inner Ear Disorders
  • 発表場所: Kyoto, Japan
  • 年月日: 2010-11-16
• [学会発表] Vibration Dynamics of Bionic Auditory Membrane for a Novel Artificial Cochlea (2010)
  • 著者名/発表者名: Hirofumi Shintaku and Satoyuki Kawano, Takayuki Nagawa, and Juichi Ito
  • 学会等名: Proceedings of Sixth International Symposium on Meniere's Disease and Inner Ear Disorders
  • 発表場所: Kyoto, Japan
  • 年月日: 2010-11-16
• [学会発表] Measurement of Electrically Evoked Auditory Brainstem Response Using Bionic Auditory Membrane with Frequency Selectivity (2010)
  • 著者名/発表者名: Hirofumi Shintaku, Takatoshi Inaoka, Yohei Nakamoto, Masahide Hayashi, Yoichi Kagaya, Takayuki Nakagaya, Juichi Ito, and Satoyuki Kawano
  • 学会等名: Technical Digest of the 5th Asia-Pacific Conference on Transducers and Micro-Nano Technology
  • 発表場所: University of Western Australia, Perth, Australia
  • 年月日: 2010-07-07
• [備考] 2011年8月1日 平成23年 大阪大学功績賞(新宅博文)
• [備考] 2010年4月23日日本機械学会奨励賞(研究), MEMS 流体デバイスによる界面現象の制御と計測に関する研究(新宅博文)