| 研究課題/領域番号 |
22K06430
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| 研究機関 | 京都大学 |
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研究代表者 |
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| 研究分担者 |
土井 大輔 京都大学, iPS細胞研究所, 特定拠点講師 (10587851)
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| 研究期間 (年度) |
2022-04-01 – 2025-03-31
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| キーワード | Parkinson's Disease / Magnetic Nanoparticle / Cell Replacment / Stem Cells / Dopaminergic Neurons |
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| 研究実績の概要 |
In the FY2023, large efforts were made towards the magnetic manipulation of nanoparticle-loaded dopaminergic neurospheres. In close collaboration with the Raffa Lab (Pisa, Italy) we were able to show the asymmetric neurite growth in direction of the magnetic field.
As the transition to animal models requires further optimization, we performed the magnetic manipulation in ex vivo cultures. These cultures are 350 um thick sections of the mouse brain. Nanoparticle=loaded spheres were grafted into these ex vivo cultures. We were able to see neurite growth in the direction of the magnetic field.
In summary, in the FY2023, we were able to translate our approach to the neurosphere model and were able to show that the stretch growth is still presence in an organotypic environment.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
As mentioned above, we were able to tackle the two major points proposed for FY2023. On the one hand, the magnetic manipulation of neurospheres was done. On the other hand, the first step towards the application of our approach in vivo was tested. Now, the actual application in vivo can be tested.
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| 今後の研究の推進方策 |
The first step is generating more data with our neurosphere and ex vivo ex vivo model. After optimizing the parameters, we can translate the approach to the animal model. Here, the biggest task is to finalize designing an appropriate magnetic applicator.
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| 次年度使用額が生じた理由 |
The animal experiments are scheduled for the upcoming fiscal year and therefore more costs will be generated. Budget is carried over to cover these costs.
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