創薬用複合型心筋組織電学機能評価システムの開発

2022 年度 実施状況報告書

• 研究課題/領域番号: 22K12801
• 研究機関: 大阪大学
• 研究代表者: 李 俊君 大阪大学, 大学院医学系研究科, 特任助教 (10723786)
• 研究分担者: 劉 莉 大阪大学, 大学院医学系研究科, 特任准教授 (50380093)
• 研究期間 (年度): 2022-04-01 – 2025-03-31
• キーワード: Pluripotent stem cells / Cardiomyocytes / Maturation / Drug assessment

研究実績の概要

We planned to reproduce the traveling wave on 2D plate and optimize the best condition for obtain matured hiPSC derived cardiomyocytes, to compare the traveling wave matured cardiomyocytes with adult cells. The achievements in the year include: 1. The traveling wave has been generated in cardiac tissue cultured on both plate and nanofiber sheets. The electrical signal of cardiac tissue could be recorded by using MEA. 2.The maturity of traveling wave matured cardiomyocytes has been confirmed by using staining, TEM, RNAsequencing and other methods. Their maturation level has been compared with those from human adult. 3. We published 2 papers and gave an oral presentation in 87th Annual Scientific Meeting of the Japanese Circulation Society, and obtained the young investigator award.

現在までの達成度 (区分)

2: おおむね順調に進展している

理由

We have accomplished the objective of the first year rather smoothly. Now we have already been working according to second year plan.

今後の研究の推進方策

In this year, we will focus on integrating the traveling wave matured cardiac tissue on a multi electrode array (MEA) system. The drug test will also be performed on the matured tissue with MEA system. The tissue culture method and the drug test condition will be optimized. The data collected during experiment will be summarized to prepare papers.

研究成果

• [雑誌論文] Tissue Sheet Engineered Using Human Umbilical Cord-Derived Mesenchymal Stem Cells Improves Diabetic Wound Healing (2022)
  • 著者名/発表者名: Zhang Jingbo、Qu Xiang、Li Junjun、Harada Akima、Hua Ying、Yoshida Noriko、Ishida Masako、Sawa Yoshiki、Liu Li、Miyagawa Shigeru
  • 雑誌名: International Journal of Molecular Sciences 巻: 23 ページ: 12697～12697
  • DOI: 10.3390/ijms232012697
• [雑誌論文] Engineered Tissue for Cardiac Regeneration: Current Status and Future Perspectives (2022)
  • 著者名/発表者名: Li Junjun、Liu Li、Zhang Jingbo、Qu Xiang、Kawamura Takuji、Miyagawa Shigeru、Sawa Yoshiki
  • 雑誌名: Bioengineering 巻: 9 ページ: 605～605
  • DOI: 10.3390/bioengineering9110605
• [学会発表] Human iPSC-derived closed-loop cardiac tissue for improved drug assessment (2023)
  • 著者名/発表者名: Ying Hua, Junjun Li, Xiang Qu, Jingbo Zhang, Masako Ishida, Noriko Yoshida, Hayato Miyoshi, Mikio Shiba3, Shuichiro Higo4,5, Nagako Sougawa1, Maki Takeda1, Takuji Kawamura1, Ryohei Matsuura, Toshihiko Toyofuku, Yoshiki Sawa, Li Liu and Shigeru Miyagawa
  • 学会等名: The 87th Annual Scientific Meeting of the Japanese Circulation Society (JCS2023)
  • 招待講演