| 研究課題/領域番号 |
22K12822
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| 研究機関 | 熊本大学 |
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研究代表者 |
Lee Ruda 熊本大学, 産業ナノマテリアル研究所, 准教授 (00802050)
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| 研究期間 (年度) |
2022-04-01 – 2025-03-31
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| キーワード | size changeable / breast cancer / nanoparticles |
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| 研究実績の概要 |
In FY2022, I planned to develop and optimize the polymer characteristic. I modified D-α-Tocopherol polyethylene glycol 1000 succinate (TPGS) with EGF receptor-targeted peptide and evaluated the conjugation. I successfully prepared the TPGS-EGF polymer and formulated drug-loaded micelles. We confirm the micelles' characteristics are suitable to move next step.
To deliver the micelles directly into the nucleus, we capped the micelles using a 150 nm size liposome. As a result, about ten micelles are captured in the liposome, and the drug loading efficacy was increased up to 4 times that of the micelles. The liposome included pH-sensitive moieties, and the release profile started to confirm.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
The chemical conjugation succeeded and formed the ideal size of micelles and liposomes. Furthermore, the drug loading efficiency is 6% in micelles and 14% in liposomes. The size controls, and drug loading efficacy was available so that the research could move to the next step in FY2023.
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| 今後の研究の推進方策 |
In FY2023, I will set the drug release profile under pH 6.7 and pH 7.4. At the same time, the nanoparticles in vitro characterization will be done in the multi-drug resistance-induced triple-negative breast (TNBC) cell line. To form a similar cancer condition, I will use microfluidics and a spheroid system. For moving to the animal experiment, I will prepare the animal protocol and will get approval from the IACUC of Kumamoto University.
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