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2022 年度 実施状況報告書

Development of online algae counting technique for avoiding musty odor in drinking water

研究課題

研究課題/領域番号 22K14352
研究機関長崎大学

研究代表者

ブアバー サンドリンルーシーパスカル  長崎大学, 工学研究科, 特任研究員 (80896704)

研究期間 (年度) 2022-04-01 – 2025-03-31
キーワードdrinking water / algae / odor / monitoring
研究実績の概要

The criteria (dimensions, shape, fluorescence intensity) for automatically identifying one odor-producing algae (2-MIB producing cyanobacteria) was established for quantification using phycocyanin auto-fluorescence. The use of a phycocyanin filter allows to separate cyanobacteria from the rest of algae. As a result, the accuracy for quantifying the concentrations of odor-producing algae has been considerably improved. Different flow cells for feeding samples were tested to automate the analysis and sample preparations. A suitable flow cell was integrated into the microscope. Different flux were tested to avoid algae clusters. The pump was programmed with different intervals to allow for algae settling before image capture. By using a programmed pump and the time-lapse capture module of the microscope, it was possible to take images of the samples automatically.

現在までの達成度 (区分)
現在までの達成度 (区分)

2: おおむね順調に進展している

理由

The criteria (dimensions, shape, fluorescence intensity) for automatically identifying one odor-producing algae was established. To automate the analysis and sample preparations, a flow cell was successfully integrated into the microscope. By combining a programmed pump and the time-lapse capture module of the microscope, automated captures were successful. That is suitable for continuous monitoring. Data collection for other algae has started.

今後の研究の推進方策

The data collection for other types of algae will continue. The criteria for automatically counting other algae causing problems for the drinking water treatment plant will established.

  • 研究成果

    (1件)

すべて 2023

すべて 学会発表 (1件)

  • [学会発表] Monitoring of 2-MIB Producing Cyanobacteria (Pseudanabaena sp.) in Drinking Water Sources by Automated Cell Imaging Using Phycocyanin Fluorescence2023

    • 著者名/発表者名
      Sandrine Boivin; Takahiro Fujioka; Eri Hasegawa; Dabide Yamaguchi
    • 学会等名
      第57回日本水環境学会年会

URL: 

公開日: 2023-12-25  

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