| 研究実績の概要 |
Multiple lines of evidence documented from functional neuroimaging, pharmacological and lesion studies, and computational modeling illustrate the involvement of striatal acetylcholine (ACh) in forms of flexible behaviours such as reversal learning and set-shifting. Despite this emerging framework, there has not been any direct recording of the spatiotemporal dynamics of ACh in behavioural tasks requiring flexible responding. To address this, I designed a closed-loop virtual-reality response learning task for head-fixed mice using the jetball system. To characterize ACh activity, I have commenced in vivo imaging using 2-photon microscopy via a GRIN lens implanted above the striatum and a genetically encoded biosensor that permits ultrafast cellular resolution imaging of ACh, iAChSnFR.
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