| 研究課題/領域番号 |
22K15656
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| 研究機関 | 兵庫医科大学 |
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研究代表者 |
段 ショウキ 兵庫医科大学, 医学部, 助教 (20910607)
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| 研究期間 (年度) |
2022-04-01 – 2025-03-31
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| キーワード | SNS / IBS / Micro-inflammation / Visceral pain |
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| 研究実績の概要 |
This year, I primarily conducted genetic modulation experiments. Using DBH-cre mice and AAV-PHP.s-Gq, I successfully activated sympathetic nervous system (SNS) activity through CNO injection, as confirmed by double immunohistochemical staining of cre and mcherry, and the NE concentration in the serum. The results indicated that immune cells were recruited into the GI tract following SNS activation, consistent with findings in the MS model. As for the underlying mechanisms, I checked several cytokines and chemokines, and aims to clarify the connection between SNS activity and immune alteration.
I attended two academic conferences this year. I did symposium speaking in the 46th Annual Meeting of the Japan Neuroscience Society. And I did an oral presentation in the 45th Annual Meeting of Japanese Association for the Study of Pain.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
I proved that activate SNS recruited immune cells into the GI tract using genetic modulation method. By those data, I achieved the second step of the project.
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| 今後の研究の推進方策 |
In the next year, I will clarify the underlying mechanism that SNS modulates the immune cell immigration. Especially the cytokines, chemokines and related cell type. In addition, I will summarize the data and prepare the manuscript to publish the article.
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| 次年度使用額が生じた理由 |
I am planning to attend an international conference next year, so I have allocated some budget for it. Regarding the experiment, the funds will be utilized for the procurement of animals, ELISA kits, and consumables. I will summarize the data in preparation for publication of the article. Additionally, the funds will cover the publishing fees.
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