| 研究実績の概要 |
We discovered that HBV infection can influence the expression of certain genes related to ferroptosis in both HBV-infected and uninfected groups. In 2023, our goal is to explore the role of these genes in HBV infection. Using a loss-of-function approach with siRNA, we observed that reducing a ferroptosis biomarker in an HBV-infected cell line (NTCP-HepG2) led to the following results: 1) a slight increase in cell viability; 2) a decrease in HBV markers such as HBV DNA, HBsAg, and HBeAg. Subsequently, we used an inhibitor targeting a ferroptosis biomarker to validate the siRNA results, which showed similar trends. Additionally, the phenotypic detection of ferroptosis indicated that the siRNA-transfected group exhibited a significant decrease in lipid ROS compared to the NC group.
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| 今後の研究の推進方策 |
In 2024, our goal is to comprehend the function of this gene in living organisms. To achieve this, we will use the hydrodynamic injection (HDI) HBV replication mice model. We will deliver either the siRNA or the inhibitor of the target gene. Subsequently, we will quantify markers of ferroptosis and HBV infection to the HDI HBV to gain a better understanding of the role of this gene.
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