| 研究課題/領域番号 |
22K17259
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| 研究機関 | 北海道医療大学 |
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研究代表者 |
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| 研究期間 (年度) |
2022-04-01 – 2024-03-31
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| キーワード | Amleogenin / Clone / Malassez |
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| 研究実績の概要 |
Epithelial cell rests of Malassez (ERM) are an easy source of stem cell-like cells. Our previous work has successfully demonstrated that an ERM variant (isolated clone of ERM) can maintain healthy periodontal space by inhibiting ankylosis between the tooth and alveolar bone.
This study aims to use the applicant's previously isolated clone model to repair the lost or injured periodontal ligament (PDL) tissue to restore tooth form and function.
Phase-1: We obtained primary ERM from PDL tissues of porcine and isolated clones of ERM. We isolated clones 1-10. The characterization was done using immunofluorescence staining of ERM cell markers.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
We have already isolated clones and characterized their origin as Epithelial cell rests of malassez markers.
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| 今後の研究の推進方策 |
Phase-2: ERM clones will be checked for enamel matrix proteins and some stem cell markers.
Pilot experiments to combine some cells to produce cell sheets.
Phase-3: Histological study of the transplanted tooth. Next-generation sequencing of ERM clones with stem cell-like properties and analysis to identify essential genes.
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| 次年度使用額が生じた理由 |
Delays in delivery and price increases for required research reagents resulted in insufficient funds remaining to be used in the next fiscal year.
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