Establishing a low-cost next-generation sequencing platform for on-site real-time surveillance of SARS-CoV-2 and other viruses in an unreached community from the Democratic Republic of Congo

2022 年度 実施状況報告書

• 研究課題/領域番号: 22K17352
• 研究機関: 大阪公立大学
• 研究代表者: チバング・カバンバ エバリステ 大阪公立大学, 大学院医学研究科, 特任助教 (50900693)
• 研究期間 (年度): 2022-04-01 – 2024-03-31
• キーワード: SARS-CoV-2 / Virus / Congo / Sequencing / Genome / Mbujimayi

研究実績の概要

In the FY2022, we developed the portable viral surveillance toolkit and obtained various research authorizations. Therefore, we brought together needed equipment and reagents to set up and validate the portable toolkit fitted in a suitcase for low-cost and rapid sequencing of SARS-CoV-2 and other viruses circulating in different regions, including rural areas of the Democratic Republic of Congo (DRC). Crucial steps were taken to obtain research agreements including collaboration and material transfer agreements between Japanese and Congolese universities as well as the ABS permit by Congolese Officials. Finally, research protocols for field studies were drafted and submitted for approval to the National Ethics Committee of the Ministry of Health in the DRC.

現在までの達成度 (区分)

2: おおむね順調に進展している

理由

The project experienced slight delays in receiving reagents ordered from outside of Japan, as well as in administrative procedures for obtaining authorizations from Congolese officials. Restrictions related to the COVID-19 pandemic situation also played some role in slowing activities. However, at this stage, the progress made is encouraging and suggests that the projects can be completed within the timeframe set by the grant.

今後の研究の推進方策

The next phase of this project consists of the field deployment of the viral surveillance toolkit to conduct molecular surveys in rural DRC from July to September 2023. These surveys will sequence viruses possibly circulating among Congolese people and will also investigate the potential mother-to-child transmission of different viruses. In subjects positive for specific viruses such as SARS-CoV-2, Mpox, and hepatitis B virus, additional analyzes will be carried out to characterize the viral variants involved. Outcomes obtained will be discussed with local health-decision makers for early response to endemic viral infections and will be published as scientific articles in international journals.

次年度使用額が生じた理由

The amount for the next fiscal year will mainly be used for deploying the viral surveillance toolkit in DRC to investigate outbreaks and for reporting research outcomes. Therefore, consumables and reagents will be purchased. Then an investigation trip will be conducted to the study area from July to September 2023. The study populations will be gathered, examined, and tested. Viral infections detected will be sequenced, analyzed, and reported.

研究成果

• [国際共同研究] University of Mbujimayi/INRB(コンゴ民主共和国)
  • 国名: コンゴ民主共和国
  • 外国機関名: University of Mbujimayi/INRB
• [雑誌論文] Age-adjusted impact of prior COVID-19 on SARS-CoV-2 mRNA vaccine response (2023)
  • 著者名/発表者名: Nakagama Sachie、Nakagama Yu、Komase Yuko、Kudo Masaharu、Imai Takumi、Tshibangu-Kabamba Evariste、Nitahara Yuko、Kaku Natsuko、Kido Yasutoshi
  • 雑誌名: Frontiers in Immunology 巻: 14 ページ: -
  • DOI: 10.3389/fimmu.2023.1087473
• [雑誌論文] Antibody Avidity Maturation Following Recovery From Infection or the Booster Vaccination Grants Breadth of SARS-CoV-2 Neutralizing Capacity (2022)
  • 著者名/発表者名: Nakagama Y, Candray K, Kaku N, Komase Y, Rodriguez-Funes MV, Dominguez R, Tsuchida T, Kunishima H, Nagai E, Adachi E, Ngoyi DM, Yamasue M, Komiya K, Hiramatsu K, Uemura N, Sugiura Y, Yasugi M, Yamagishi Y, Mikamo H, Shiraishi S, Izumo T, Nakagama S, Watanabe C, Nitahara Y, Tshibangu-Kabamba E, Kakeya H, Kido Y.
  • 雑誌名: The Journal of Infectious Diseases 巻: 227 ページ: 780～787
  • DOI: 10.1093/infdis/jiac492
• [雑誌論文] A Dual-Antigen SARS-CoV-2 Serological Assay Reflects Antibody Avidity (2022)
  • 著者名/発表者名: Nakagama Yu、Nitahara Yuko、Kaku Natsuko、Tshibangu-Kabamba Evariste、Kido Yasutoshi
  • 雑誌名: Journal of Clinical Microbiology 巻: 60 ページ: -
  • DOI: 10.1128/JCM.02262-21
• [雑誌論文] Cumulative seroprevalence among healthcare workers after the first wave of the COVID-19 pandemic in El Salvador, Central America (2022)
  • 著者名/発表者名: Nakagama Yu、Rodriguez-Funes Maria-Virginia、Dominguez Rhina、Candray-Medina Katherine-Sofia、Uemura Naoto、Tshibangu-Kabamba Evariste、Nitahara Yuko、Kaku Natsuko、Kaneko Akira、Kido Yasutoshi
  • 雑誌名: Clinical Microbiology and Infection 巻: 28 ページ: 1508～1510
  • DOI: 10.1016/j.cmi.2022.06.020
• [雑誌論文] Detecting Waning Serological Response with Commercial Immunoassays: 18-Month Longitudinal Follow-up of Anti-SARS-CoV-2 Nucleocapsid Antibodies (2022)
  • 著者名/発表者名: Nakagama Yu、Komase Yuko、Kaku Natsuko、Nitahara Yuko、Tshibangu-Kabamba Evariste、Tominaga Tomoyo、Tanaka Hiroko、Yokoya Tomoaki、Hosokawa Minako、Kido Yasutoshi
  • 雑誌名: Microbiology Spectrum 巻: 10 ページ: -
  • DOI: 10.1128/spectrum.00986-22
• [学会発表] Tungiasis in rural communities from the Democratic Republic of Congo: Burden, clinical impact and local disease's determinants (2022)
  • 著者名/発表者名: Evariste Tshibangu-Kabmba
  • 学会等名: 第92回日本寄生虫学会大会