| 研究課題/領域番号 |
22K20535
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| 研究機関 | 岡山大学 |
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研究代表者 |
FERREPUJOL PILAR 岡山大学, 異分野融合先端研究コア, 特任助教 (50839008)
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| 研究期間 (年度) |
2022-08-31 – 2024-03-31
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| キーワード | graphene oxide / reduced graphene oxide / oocyte / in vitro maturation |
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| 研究実績の概要 |
We prepared graphene oxide (GO) according to a previous method (Hummer’s method) and slowly performed thermal reduction at 80°C up to 2 weeks to ensure a successful reduction of the functional groups to obtain reduced graphene oxide (rGO). The GO and rGO samples were structurally analyzed by FTIR, TGA, XPS and XRD. To analyze their reactive oxygen species (ROS) activity, they were analyzed by ESR. Furthermore, to understand their ability to be used in a culture medium setting, the samples were submitted to a dispersion test.
According to the results of all the tests, pristine GO (oxidation level 30-35%) was selected to analyze its toxicity during oocyte in vitro maturation (IVM) in the presence of non-enzymatic antioxidants. Results suggest that the addition of GO during oocyte IVM does not negatively affect oocyte viability significantly.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
We have finalized stage one of our research, related to the preparation and analysis of GO and rGO. Furthermore, we analyzed the safety to use this material in an in vitro setting.
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| 今後の研究の推進方策 |
We intend to further analyze the effect of GO on oocyte IVM in the absence of antioxidants, to assess its use as a ROS quencher to protect the oocytes in an in vitro setting from oxidative stress.
Depending on the results of the experiment, we are also planning to assess the effect of GO addition during oocyte vitrification, as well as after oocyte vitrification, during the recovery stage, to see if it can reduce oocyte damage by reactive oxygen species (ROS) after thawing.
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