| 研究実績の概要 |
The results indicated that when graphene oxide (GO) and reduced graphene oxide (rGO) were used during vitrification, they negatively affected the survival of cumulus-oocyte-complexes due to the strong affinity of the materials with the cell membranes. Furthermore, when the materials were added after thawing, their protective effect against reactive oxygen species could also not be clearly evaluated. The high reactivity of the surface of GO with the inorganic components of the culture media (especially with positive ions such as Na, K or Ca) could partly explain the reason why pristine GO and rGO were unable to successfully protect the oocytes from oxidative stress.
Further studies employing surface-modified GO are necessary to successfully use this material for cryopreservation.
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