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The purpose of this research is two-fold: 1.Evaluate the regulation of energy homeostasis by the skeleton (bone) and 2.The effect of energy perturbation on the skeleton. We developed Glut1 bone specific knockout mice to study the effect of glucose abstinence on bone structural and functional parameters. Mice body weight decreased in the knockout group which indicates that glucose metabolism by the skeleton affects whole body metabolism and bone density. Additionally transcriptome and proteome-wide analysis conducted on hyperglycemic osteocytes (bone cells) were used to constructing a molecular network to reveal a bone-specific effect of hyperglycemia across the analyzed omics levels which revealed a dissociation between transcription (gene expression) and translation (osteokine levels).
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