| 研究課題/領域番号 |
22K20705
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| 研究機関 | 北海道大学 |
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研究代表者 |
タン ロジャー・サルバシオン 北海道大学, 先端生命科学研究院, 助教 (10951759)
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| 研究期間 (年度) |
2022-08-31 – 2024-03-31
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| キーワード | Exosome-nanosme / Exosome / Cancer cells / Nanoparticle / Nanosome |
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| 研究実績の概要 |
During the first fiscal year of research implementation, production and isolation have been optimized to yield high number of exosomes. This part is important for the incorporation of "nanosome" as the number of "exosome-nanosome" platform to be produced is dependent on the produced exosomes. The amount of exosome produced between fed and starved cells were also determined to be different. Some exosome proteins are downregulated in the starved state. These are significantly important as the "exosome-nanosome" incorporation on different cells/organs can also be impacted significantly.
The construction of the platform showed limitations on the incorporation of the "nanosome" and optimization (sizes, ligand composition, and the overall charge) seemed to be necessary.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
3: やや遅れている
理由
External Factor (Uncontrolled):
Reagent purchasing challenges were encountered. There are reagent, antibodies, gels, filters, and column, that are not available in Japan and have to be imported from abroad. Delivery of these reagents usually takes 4-6 weeks.
Internal Factor (Controlled):
During the implementation, challenges and hurdles that require optimization
steps were encountered. These optimization trials for one parameter usually takes a minimum of 2-3 weeks to complete.
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| 今後の研究の推進方策 |
To mitigate the problems on longer delivery time of reagents, bulk ordering will be followed. The next steps will be finishing optimizations, especially on the construction of the platform. After the construction, biodistribution assays will follow, and then drug loading and efficiency testing. To push forward the progress of the research, collaboration (at least personal level) will be sought as well.
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| 次年度使用額が生じた理由 |
This will be used to purchase used up reagents and disposable items.
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