| 研究課題/領域番号 |
21F21379
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| 配分区分 | 補助金 |
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| 研究機関 | 東北大学 |
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研究代表者 |
植田 美那子 東北大学, 生命科学研究科, 教授 (20598726)
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| 研究分担者 |
KAO PING 東北大学, 生命科学研究科, 外国人特別研究員
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| 研究期間 (年度) |
2021-11-18 – 2024-03-31
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| キーワード | Arabidopsis thaliana / embryogenesis / transcriptome / cellular dynamics |
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| 研究実績の概要 |
This study aims to identify the key genes involved during embryogenesis in Arabidopsis thaliana (Arabidopsis). Zygotic embryogenesis is a fundamental developmental process in which a single-celled zygote goes through a series of cell divisions, patterning and cell type specifications to form a complex embryo during eukaryotic sexual reproduction. In the flowering plant Arabidopsis, the zygotes divide with a stereotypic pattern, making them an ideal system to observe embryo morphology and to trace cell lineage. While decades of genetic analyses have identified a number of genes that play vital roles in embryogenesis, the genome-wide analyses were not feasible until recently due to technical difficulties. We utilized published embryonic transcriptome datasets to search for key regulatory genes of embryogenesis as well as to resolve the underlying regulatory networks. With bioinformatics approaches, we selected a number of candidate genes for further validations and analyses. Identifying the key factors involved in embryonic cell divisions and specifications will greatly improve our knowledge of flowering plant embryogenesis mechanisms, which is important for understanding the fundamental biological process enabling multicellular eukaryotic lives.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
1: 当初の計画以上に進展している
理由
We have collected and analyzed published Arabidopsis embryonic transcriptome data and searched for promising factors contributing to crucial cellular events during zygotic embryogenesis. Several genes were selected, and the molecular and genetic examinations of these factors are now in progress.
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| 今後の研究の推進方策 |
First, reporters of the selected genes will be made, and the transgenic lines will be examined to validate the expression patterns. The confirmed genes will then be subjected to functional analyses with mutant lines and molecular variants. Additional RNA sequencing is also scheduled in order to search for more key genes involved in embryogenesis.
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