| 研究課題/領域番号 |
21F21379
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| 配分区分 | 補助金 |
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| 研究機関 | 東北大学 |
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| 受入研究者 |
植田 美那子 東北大学, 生命科学研究科, 教授 (20598726)
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| 外国人特別研究員 |
KAO PING 東北大学, 生命科学研究科, 外国人特別研究員
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| 研究期間 (年度) |
2021-11-18 – 2024-03-31
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| キーワード | Arabidopsis thaliana / zygotic embryogenesis / transcriptome / cellular dynamics |
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| 研究実績の概要 |
The goal of this study is to identify the key regulatory genes involved in embryogenesis in Arabidopsis thaliana (Arabidopsis). Zygotic embryogenesis is a fundamental developmental process in which a single-celled zygote goes through a series of cell divisions, patterning and cell type specifications to form a complex embryo during eukaryotic sexual reproduction. In the flowering plant Arabidopsis, zygotes divide stereotypically, allowing observation of embryo patterning events and tracing cell lineages. While decades of genetic analyses have identified a number of genes that play vital roles in embryogenesis, the genome-wide analyses were not feasible until recently due to technical difficulties. We utilized published embryonic transcriptome datasets to search for key regulatory genes of embryogenesis as well as to resolve the underlying regulatory networks. With bioinformatics approaches, we selected a number of candidate genes for further validations and analyses. Identifying the key factors involved in embryonic cell divisions and specifications will greatly improve our knowledge of flowering plant embryogenesis mechanisms, which is important for understanding the fundamental biological process enabling multicellular eukaryotic lives.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
We collected published embryonic transcriptome datasets (Zhao et. al. 2019, Hofmann et. al. 2019, Kao et. al. 2021) and refined analytic pipeline. Gene expression variations among different cell types or developmental stages were highlighted for subsequent analyses. A list of candidate genes that might contribute to the embryonic developmental events has been selected for further examinations. Promoter fusions of several candidate genes have been cloned and their expression patterns have been examined. The molecular and genetic examinations of the selected genes are now in progress.
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| 今後の研究の推進方策 |
Cloning of translational reporter and translational fusion with activators or repressors is ongoing. In addition, we will incorporate more datasets and refine analytic pipelines in attempt to better identify key regulatory genes.
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