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2022 年度 実績報告書

PilVax経鼻インフルエンザワクチンの有効性の評価

研究課題

研究課題/領域番号 22F20801
配分区分補助金
研究機関千葉大学
受入研究者 藤橋 浩太郎  千葉大学, 医学部附属病院, 特任教授 (50820354)
外国人特別研究員 TSAI JIA-YUN  千葉大学, 医科学研究所, 外国人特別研究員
研究期間 (年度) 2022-07-27 – 2024-03-31
キーワードNasal Vaccine / Influenza virus / IgA / Protection
研究実績の概要

Age-matched female BALB/c mice were immunized with of PilVax vaccines (1 X 10^8 CFU, in a volume of 10 ul) presenting two tandem copies (M2e-M2e-PilVax) of the M2e peptide. The vaccines were administered on 3 consecutive days with additional 3 boosters given 2 weeks apart, for a total of 12 doses. One week after the final immunization (day 51 or 49), serum, bronchoalveolar lavage (BAL) nasal washes (NWs) and saliva were collected and subjected to M2e-specific ELISAs. Significantly increased levels of M2e-specific serum IgG and mucosal IgA antibody responses were noted.

現在までの達成度 (区分)
現在までの達成度 (区分)

2: おおむね順調に進展している

理由

Age-matched female BALB/c mice were immunized with of PilVax vaccines (1 X 10^8 CFU, in a volume of 10 ul) presenting two tandem copies (M2e-M2e-PilVax) of the M2e peptide. The vaccines were administered on 3 consecutive days with additional 3 boosters given 2 weeks apart, for a total of 12 doses. One week after the final immunization (day 51 or 49), serum, bronchoalveolar lavage (BAL) nasal washes (NWs) and saliva were collected and subjected to M2e-specific ELISAs. Significantly increased levels of M2e-specific serum IgG and mucosal IgA antibody responses were noted.

今後の研究の推進方策

Tissue samples will be isolated from nasal mucosa and lungs and subjected to immunohistochemical analyses by using confocal laser scanning microscopy in order to characterize the distribution of dendritic cells, T and B cells. Furthermore, we will test the functional properties of the M2e-specific antibodies by challenging vaccinated mice with influenza virus strains (H1N1, e.g., A/California and/or H3N2, e.g., A/Guizhou) to confirm protective immunity.

  • 研究成果

    (1件)

すべて 2023

すべて 雑誌論文 (1件) (うち査読あり 1件、 オープンアクセス 1件)

  • [雑誌論文] Mucosal vaccination: onward and upward2023

    • 著者名/発表者名
      Tsai Catherine J. Y.、Loh Jacelyn M. S.、Fujihashi Kohtaro、Kiyono Hiroshi
    • 雑誌名

      Expert Review of Vaccines

      巻: 22 ページ: 885~899

    • DOI

      10.1080/14760584.2023.2268724

    • 査読あり / オープンアクセス

URL: 

公開日: 2024-12-25  

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