| 研究課題/領域番号 |
22F22054
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| 配分区分 | 補助金 |
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| 研究機関 | 岐阜大学 |
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研究代表者 |
李 富生 岐阜大学, 流域圏科学研究センター, 教授 (10332686)
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| 研究分担者 |
BHAT SARTAJ 岐阜大学, 流域圏科学研究センター, 外国人特別研究員
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| 研究期間 (年度) |
2022-09-28 – 2025-03-31
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| キーワード | Earthworms / microplastics / cow dung / microbes / vermicomposting |
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| 研究実績の概要 |
Vermireactors were used for treating three different concentrations (0.5%, 1% and 2%) of LDPE and PBSA of <2 mm size with the addition of cow dung (CD). The CD supported the growth and hatchlings of earthworms, and the toxicity effect of both LDPE and PBSA microplastics on earthworm Eisenia fetida was analysed. For vermicomposting, the mixtures were kept for thermo stabilization for at least a week. After that, clitellated Eisenia fetida species of earthworms were incubated separately @ 5 worms’ 250 g-1 of the CD for a period of 120 days. Experimental samples of both CD and earthworms were collected at 60 and 120 days of vermicomposting and analyzed for various physico-chemical parameters, like the moisture content measured by drying the samples to a constant weight at 105 °C (24 h) in an oven. The organic matter content was determined by combusting the dried samples in a muffle furnace at 600 °C for 2 h. The pH and electrical conductivity (EC) were also determined by using the aqueous solution of dry sample with deionized water (w/v = 1/10) after shaken for 2 h. Scanning Electron Microscopy (SEM), and Fourier Transform Infrared Spectroscopy (FTIR) analysis were also conducted following standard protocols. The initial results suggested that the LDPE microplastics pose more threat to earthworm Eisenia fetida as compared to the PBSA microplastics.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
We have completed the vermicomposting part of the study for 120 days of treatment period. The study is going according to the plan, and we will further study the samples for their physico-chemical analysis and microbial analysis.
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| 今後の研究の推進方策 |
The next plan for the study will to be conduct the microbial analysis of the samples using 16srDNA by the quantitative PCR method. The physico-chemical analysis for the 120 day of vermicomposting samples will also be conducted. After that all data of vermicomposting, SEM, FT-IR and microbial analysis will be compiled for the writing of the research paper.
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