• 研究課題をさがす
  • 研究者をさがす
  • KAKENの使い方
  1. 課題ページに戻る

2022 年度 実績報告書

微小管のメカノトランスダクション機能に関する包括的な理解

研究課題

研究課題/領域番号 22F22050
配分区分補助金
研究機関京都大学

研究代表者

角五 彰  京都大学, 理学研究科, 教授 (10374224)

研究分担者 NASRIN SYEDA  京都大学, 理学(系)研究科(研究院), 外国人特別研究員
研究期間 (年度) 2022-09-28 – 2025-03-31
キーワードKinesin / Dynein / Microtubule / Mechanical stress / Mechano-transducer
研究実績の概要

The current research hypothesizes that microtubules are mechanotransducers of mechanical stress. In this study, a comprehensive understanding of the issue will be made. Last year the modulation of cargo transport in response to mechanical stress on the microtubule is a universal phenomenon independent of the motor protein by using kinesin was verified. Kinesin-driven transport of fluorescent quantum dots was carried out on microtubules fixed on a stretchable substrate. The effect of the mechanical stress on the cargo transport was systematically evaluated by applying compressive strain or tensile strain using the microstretcher chamber developed previously. Parameter analysis e.g., run length, velocities, pause frequencies, etc., from the fluorescence microscopy observation was done.

現在までの達成度 (区分)
現在までの達成度 (区分)

2: おおむね順調に進展している

理由

Because the research is progressing as planned, it was concluded that the project was on track.

今後の研究の推進方策

This year, we will verify if the modulation of cargo transport in response to mechanical stress is recovered if the applied stress on the microtubule is withdrawn. For this research, I will first apply compressive stress on the microtubule using my micro-stretcher and release the stress. Then I will observe dynein or kinesin-driven cargo transport and compare it with the case of undeformed microtubules to explore the restoration of the transport dynamics.

  • 研究成果

    (3件)

すべて 2022

すべて 学会発表 (1件) (うち国際学会 1件、 招待講演 1件) 図書 (2件)

  • [学会発表] Microtubules function as mechanosensor to regulate intracellular transport2022

    • 著者名/発表者名
      Akira Kakugo
    • 学会等名
      EMBL Symposium: Microtubules: From Atoms to Complex Systems
    • 国際学会 / 招待講演
  • [図書] Methods in Molecular Biology, Microtubules-Methods and Protocols2022

    • 著者名/発表者名
      Syeda Rubaiya Nasrin, Arif Md. Rashedul Kabir, Akira Kakugo
    • 総ページ数
      11
    • 出版者
      Springer Nature
  • [図書] Methods in Molecular Biology, Microtubules-Methods and Protocols2022

    • 著者名/発表者名
      6.Syeda Rubaiya Nasrin, Farhana Afroze, Arif Md. Rashedul Kabir, Akira Kakugo
    • 総ページ数
      11
    • 出版者
      Springer Nature

URL: 

公開日: 2023-12-25  

サービス概要 検索マニュアル よくある質問 お知らせ 利用規程 科研費による研究の帰属

Powered by NII kakenhi