| 研究課題/領域番号 |
21F21771
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| 配分区分 | 補助金 |
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| 研究機関 | 大阪大学 |
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研究代表者 |
藤田 克昌 大阪大学, 工学研究科, 教授 (80362664)
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| 研究分担者 |
LINDLEY MATTHEW 大阪大学, 工学(系)研究科(研究院), 外国人特別研究員
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| 研究期間 (年度) |
2021-11-18 – 2024-03-31
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| キーワード | Raman / Microplastics |
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| 研究実績の概要 |
We have tested the detectability of various microplastics in tissues using microplastic beads placed in mouse liver. Based on these tests, we have made design changes to our Raman line-scan microscope to optimize sample throughput. For producing weathered microplastics, we created a UV exposure apparatus, with four of six different types of microplastics now weathered. Test scans of mouse liver slices from our models have also yielded a bit of serendipity; in addition to assessing tissue health through cytochrome c detection, we tentatively have also detected a secondary biomarker in retinol that should serve as a reporter of liver health. Mouse models have been prepared, with the first microplastic doses using commercial samples to begin shortly.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
A UV photoreactor for plastic weathering was designed under budget and is working smoothly. We are now waiting on SEM-EDX user training (May) to begin characterization of weathered microplastics.
There has been a slight delay in initial microplastic dose-tests with the mice due to availability of commercial fluorescent microplastic samples that are within budget, but we have overcome this.
Plastic detectability tests have been successful and show project measurement is compatible with our microscope.
Our biological collaborators have been a great help in planning and scientific discussion and have afforded us prompt access to fresh tissue samples.
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| 今後の研究の推進方策 |
In R4, we will next test the distribution of microplastics in the mouse following ingestion using fluorescent microplastics and flow cytometry. We will typify the weathering results for our plastic samples. We will implement the planned changes to our rapid-scan Raman microscope and begin measurement of organ slices from microplastic-dosed mice.
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