| 研究実績の概要 |
Indian mustard (Brassica juncea) is an economically important oilseed crop in India and its production is strongly dependent on boron (B) supplies. Previously, we have hydroponically screened 26 genotypes of Indian mustard in low B (0.46μM) and sufficient B (46 μM) and identified three genotypes Geeta, RH06 and Maya as resistant, moderately resistance and sensitive to low B stress respectively. To understand the molecular mechanism of B transport in Indian mustard, we identified 6 AtBOR1, 2 AtBOR2 and 5 At NIP5;1 homologous genes in Indian mustard genome. We prepared the genomic constructs of 6 BOR1 and 2 BOR2 fused with coding sequence of GFP at 3’ end and used for cellular localization and complementation analysis. These BOR1 and BOR2 constructs were transformed into Agrobacterium rhizogenes mediated hairy roots of Indian mustard and cellular localization were analyzed. BOR2B (BjuVB06G52760) showed polar localization in the plasma membrane of epidermis, epidermis adjacent cell layer of cortex, and endodermis. Also, we have transformed 6 BOR1 and 2 BOR2 in single and double mutant of Arabidopsis BOR1 and BOR2 genes for complementation analysis and T2 seeds were harvested. We also cloned the CDS of 6 BOR1, 2 BOR2 and 5NIP5;1 from Geeta and Maya in yeast expression vector to measure boron transport activity.
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