| 研究課題/領域番号 |
21F21410
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| 配分区分 | 補助金 |
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| 研究機関 | 慶應義塾大学 |
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研究代表者 |
岡野 栄之 慶應義塾大学, 医学部(信濃町), 教授 (60160694)
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| 研究分担者 |
LEVENTOUX NICOLAS 慶應義塾大学, 医学部(信濃町), 外国人特別研究員
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| 研究期間 (年度) |
2021-09-28 – 2024-03-31
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| キーワード | Kii ALS/PDC / astrocytes / MAPT isoforms |
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| 研究実績の概要 |
I included more control-iPast samples to strengthen the RNA-seq data. We confirmed the relevance of one gene involved in the mitochondrial respiratory chain, which leads us to consider a direct role of astrocytes in the development of the pathology. We also investigated, using human brain samples, the ratio 3R/4R of MAPT transcription by ddPCR and found that in Kii-ALS, patients have a ratio value less than 1. This result needs to be confirmed using biofluids and would be useful for patient stratification.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
Our research on the Kii disease will result in the submission of 2 papers before the end of the calendar year. The paper on the generation of Kii-iPast cells only requires confirmation of the transcription of a few genes by RT-qPCR from the experimental point of view as well as finalization of the Gene Ontology. The paper on MAPT isoforms will be submitted after the WB have been finalised, a few experimental conditions remain to deal with.
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| 今後の研究の推進方策 |
We want to study the co-culture of Kii-iPast with neurons to investigate the effect of these patients’ astrocytes on neuron survival. I am currently in the process of generating neurons using well established protocols from the Laboratory, but still have to investigate cell culture impact on cell viability. The transplantation of neurospheres into the brains of NOD/SCID mice remains to be organised. Until now, the Rag2-/- pups and mice appeared as too fragile to survive long enough post engraftment. The neurospheres are currently frozen.
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