| 研究実績の概要 |
This study aimed to find an efficient method to generate tissue-type plasminogen activator (tPA) fragments, which naturally occur during tumor progression/inflammation of the hematologic cancer called multiple myeloma. To determine whether it will occur. In the first year of this proposal, we used different approaches to generate tPA fragments. Using a plasmid-based expression system in bacteria, we evaluated multiple proteases, including alpha elastase and plasmin, to cleave the purchased protein. We have successfully expressed the fragment in bacteria and are currently evaluating various methods for large-scale isolation. In one of his original articles published this year, we identified p53 as a downstream target of the small molecule and plasmin inhibitor YO2 (Cancers 15, 288; doi 10.3390/cancers15010288, 2023). A published review article summarizes the knowledge of the plasminogen-plasmin system in the oral cavity (Cells, 12(3), 445 https://doi.org/10.3390/cells12030445, 2023)."
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
We have initially used recombinant tPA and performed time course studies to identify proteases that will generate the tPA fragments. Unfortunately, non of the tested proteases was able to generate sufficient hydrolysis of the input full-length protein. Therefore, we shifted the strategy to express the tPA fragment in a plasmid. The generation of the fragment in bacteria turned out to be more difficult than expect, but we identified a strain in which the protein could be expressed. We are currently using various purification methods to obtain the protein in a tissue culture pure form.
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