| 研究実績の概要 |
Translation, as a fundamental step in gene expression, requires extensive regulation. Recent studies have shown that the trigger of ribosome-associated quality control is the collision between two translating ribosomes. However, it is not clear whether endogenous mRNA sequences could also trigger quality control.
By deep-sequencing the ribosome footprints protected by collided ribosomes prior to rescue, we determined a specific feature in the mRNA sequence that contributed to its recognition by ribosome-associated quality control. Further analysis through reporter assay also validated our findings from the analysis.
These results combined provided crucial evidences regarding co-translational quality control pathways in higher eukaryotic cells.
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