| 研究課題/領域番号 |
22J15226
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| 配分区分 | 補助金 |
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| 研究機関 | 京都大学 |
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研究代表者 |
CHENG RONG 京都大学, 工学研究科, 特別研究員(DC2)
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| 研究期間 (年度) |
2022-04-22 – 2024-03-31
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| キーワード | biometal / copper ion / cuprous ion / protein labeling / conditional proteomics / chemical proteomics |
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| 研究実績の概要 |
This year, several cuprous-ion-responsive protein labeling reagents (CuRs) have been synthesized, and the chemical structure has been optimized. The cuprous ions-responsive reactions of the optimal CuR have also been studied and validated by HPLC analysis. Moreover, the capability of CuR to label and profile proteins in response to cuprous ions in multiple living cell systems has also been confirmed by western blotting, imaging, and proteomics analysis.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
The optimal cuprous-ion-responsive protein labeling reagent (CuR) has been successfully developed. The labeling mechanism of CuR has been elucidated. CuR has also been successfully applied to multiple living cell systems. Therefore, the research has progressed on the whole more than it was initially planned.
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| 今後の研究の推進方策 |
In 2023, I will continue to collect data for the publication of the research results obtained. In particular, I will apply the developed optimal CuR to the ATP7A knock-out cells to map the proteins associated with impaired accumulation and export of copper ions by mass spectrometry to study the regulation of copper ions in living systems with genetic misregulation. After this, I will synthesize ligand-tethered cuprous ion-responsive protein labeling reagent (LT-CuR) targeting AMPA receptors to study copper release in the synapse.
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