プロトンポンプと金属イオン輸送系の機能構造、機能共役、細胞ダイナミクスの解明

2013 年度 研究成果報告書

• 研究課題/領域番号: 23248017
• 研究種目: 基盤研究(A)
• 配分区分: 補助金
• 応募区分: 一般
• 研究分野: 応用生物化学
• 研究機関: 名古屋大学
• 研究代表者: 前島 正義 名古屋大学, 生命農学研究科, 教授 (80181577)
• 研究期間 (年度): 2011-04-01 – 2014-03-31
• キーワード: 金属イオン / プロトンポンプ / イオン輸送体 / 液胞 / 分子構造 / 機能調節

研究概要

液胞膜プロトンポンプH+-PPaseと液胞膜亜鉛輸送体MTP1に注目し、MTP1の特徴的アミノ酸30残基の生化学的機能(FEBS J. 2012)、MTP1のHis 領域の分子構造と亜鉛結合性の特性(FEBS Open Bio 2013)、植物における亜鉛過剰での膜輸送系タンパク質の変動特性（Plant Physiol. 2011）、H+-PPaseのPPi加水分解機能の形態形成での重要性（Plant Cell 2011; Plant Signal. Behav. 2012）、H+-PPaseとホスファチジルイノシトールリン酸との相互作用（Plant Cell 2013）等の成果を得た。

研究成果

• [雑誌論文] The ABCA9 transporter facilitates seeds storage lipid synthesis at the endoplasmic reticulum (2013)
  • 著者名/発表者名: Kim, S., Yamaoka, Y., Ono, H., Kim, H., Shim, D., Maeshima, M., Martinoia, E., Cahoon, E.B., Nishida, I., and Lee, Y.
  • 雑誌名: Proc. Natl. Acad. Sci. U.S.A. 巻: 110 ページ: 773-778
  • 査読あり
• [雑誌論文] Arabidopsis TWISTED DWARF1 functionally interacts with auxin exporter ABCB1 on the root plasma membrane (2013)
  • 著者名/発表者名: Wang, B., Bailly, A., Zwiewka, M., Henrichs, S., Azzarello, E., Mancuso, S., Maeshima, M., Friml, J., Schulz, A., and Geisler, M.
  • 雑誌名: Plant Cell 巻: 25 ページ: 202-214
  • 査読あり
• [雑誌論文] A Ca2+-binding protein PCaP2 located on the plasma membrane is involves in root hair development as a possible signal transducer (2013)
  • 著者名/発表者名: Kato, M., Aoyama, T., and Maeshima, M.
  • 雑誌名: Plant J. 巻: 74 ページ: 690-700
  • 査読あり
• [雑誌論文] Zinc-binding and structural properties of the histidine-rich loop of Arabidopsis thaliana vacuolar membrane zinc transporter MTP1 (2013)
  • 著者名/発表者名: Tanaka, N., Kawachi, M., Fujiwara, T., and Maeshima, M.
  • 雑誌名: FEBS Open Bio 巻: 3 ページ: 218-224
  • 査読あり
• [雑誌論文] A role of phosphatidylinositol 3,5-bisphosphate in vacuolar structure change in guard cells of closing stomata (2013)
  • 著者名/発表者名: Bak, G., Lee, E.J., Lee, Y., Kato, M., Segami, S., Heven, S., Maeshima, M., Hwang, J.U., and Lee, Y.
  • 雑誌名: Plant Cell 巻: 25 ページ: 2202-2216
  • 査読あり
• [雑誌論文] Local distribution of membrane proteins on vacuoles isolated from Arabidopsis suspension-cultured cells (2013)
  • 著者名/発表者名: Yoshida, K., Ohnishi, M., Fukao, Y., Okazaki, Y., Song, C., Hayashi, F., Fujiwara, M., Nakanishi, Y., Saito, K., Shimmen, T., Suzaki, T., Fukaki, H., Maeshima, M., and Mimura, T.
  • 雑誌名: Plant Cell Physiol. 巻: 54 ページ: 1571-1584
  • 査読あり
• [雑誌論文] Mn tolerance in rice is mediated by MTP8.1, a member of the cation diffusion facilitator family (2013)
  • 著者名/発表者名: Chen, Z., Fujii, Y., Yamaji, N., Masuda, S., Takemoto, Y., Kamiya, T., Yusuyin, Y., Iwasaki, K., Kato, S., Maeshima, M., Ma, J.F., and Ueno, D.
  • 雑誌名: J. Exp. Botany 巻: 64 ページ: 4375-4387
  • 査読あり
• [雑誌論文] Enhanced cell expansion in KRP2 overexpressor is mediated through increased V-ATPase activity (2013)
  • 著者名/発表者名: Ferjani, A., Ishikawa, K., Asaoka, M., Ishida, M., Horiguchi, G., Maeshima, M., and Tsukaya, H.
  • 雑誌名: Plant Cell Physiol. 巻: 54 ページ: 1989-1998
  • 査読あり
• [雑誌論文] Class III compensation, represented by KRP2 overexpression, depends on V-ATPase activity in proliferative cells (2013)
  • 著者名/発表者名: Ferjani, A., Ishikawa, K., Asaoka, M., Ishida, M., Horiguchi, G., Maeshima, M., and Tsukaya, H.
  • 雑誌名: Plant Signaling and Behavior 巻: 8 ページ: e27204
  • 査読あり
• [雑誌論文] Regulation of pyrophosphate levels by the H+-PPase is central for proper resumption of early plant development (2012)
  • 著者名/発表者名: Ferjani, A., Segami, S., Horiguchi, G., Sakata, A., Maeshima, M., and Tsukaya, H.
  • 雑誌名: Plant Signaling & Behavior 巻: 7 ページ: 38-42
  • 査読あり
• [雑誌論文] Interaction of the trans-frame potyvirus protein P3N-PIPO with host protein PCaP1 facilitates potyvirus movement (2012)
  • 著者名/発表者名: Vijayapalani, P., Maeshima, M., Nagasaki-Takekuchi, N., and Miller, W.A.
  • 雑誌名: PLoS Pathogens 巻: 8 ページ: e1002639
  • 査読あり
• [雑誌論文] Amino acid screening based on structural modeling identifies critical residues for the function, ion selectivity and structure of Arabidopsis MTP1 (2012)
  • 著者名/発表者名: Kawachi, M., Kobae, Y., Kogawa, S., Mimura, T., Kramer, U., and Maeshima, M.
  • 雑誌名: FEBS J. 巻: 279 ページ: 2339-2356
  • 査読あり
• [雑誌論文] Stimulation of root growth induced by aluminum in Quercus serrata Thunb. is related to activity of nitrate reductase and maintenance of IAA concentration in roots (2012)
  • 著者名/発表者名: Tomioka, R., Takenaka, C., Maeshima, M., and Tezuka, T., Kojima, M., Sakakibara, H.
  • 雑誌名: American J. Plant Sciences 巻: 3 ページ: 1619-1624
  • 査読あり
• [雑誌論文] Vacuolar proton pumps and aquaporins involved in rapid internode elongation of deepwater rice (2011)
  • 著者名/発表者名: Muto, Y., Segami, S., Hayashi, H., Sakurai, J., Murai, M., Hattori, Y., Ashikari, M., and Maeshima, M.
  • 雑誌名: Biosci. Biotechnol. Biochem. 巻: 75 ページ: 114-122
  • 査読あり
• [雑誌論文] The iTRAQ analysis reveals mechanisms of growth defects due to excess zinc in Arabidopsis thaliana (2011)
  • 著者名/発表者名: Fukao, Y., Ferjani, A., Tomioka, R., Nagasaki, N., Kurata, R., Nishimori, Y., Fujiwara, M., and Maeshima, M.
  • 雑誌名: Plant Physiology 巻: 155 ページ: 1893-1907
  • 査読あり
• [雑誌論文] Chilling-induced changes of vacuolar proton pumps of hypocotyls from Vigna unguiculata (2011)
  • 著者名/発表者名: Oliveira, L.M.N., Sobreira, A.C., Sobreira, A.C.M., Costa, J.H., Otoch, M.L.O., Maeshima, M., and Fernandes de Melo, D.
  • 雑誌名: Plant Growth Regulation 巻: 64 ページ: 211-219
  • 査読あり
• [雑誌論文] Keep an eye on PPi : The vacuolar-type H+-pyrophosphatase regulates postgerminative development in Arabidopsis (2011)
  • 著者名/発表者名: Ferjani, A., Segami, S., Horiguchi, G., Muto, Y., Maeshima, M., and Tsukaya, H.
  • 雑誌名: Plant Cell 巻: 23 ページ: 2895-2908
  • 査読あり
• [雑誌論文] The small GTPase Rab5a is essential for intracellular transport of proglutelin from Golgi apparatus to the protein storage vacuole and endosomal membrane organization in developing rice endosperm (2011)
  • 著者名/発表者名: Fukuda, M., Satoh-Cruz, M., Wen, L., Crofts, A.J., Sugino, A., Washida, H., Okita, T.W., Ogawa, M., Y. Kawagoe, Y., Maeshima, M., and Kumamaru, T.
  • 雑誌名: Plant Physiol. 巻: 157 ページ: 632-644
  • 査読あり
• [学会発表] Dimerization of GFPs fused to H+-pyrophosphatase influences the morphology and dynamics of vacuoles (2013)
  • 著者名/発表者名: Segami, S. et al.
  • 学会等名: International Workshop on Plant Membrane Biology
  • 発表場所: Okayama
  • 年月日: 20130326-31
• [図書] Regulation of PPi levels through vacuolar membrane H+-pyrophosphatase. In Progress in Botany, Vol.75, ed. Luttge, Beyschlag and Cushman (2013)
  • 著者名/発表者名: Ferjani, A., Segami, S., Asaoka, M. and Maeshima, M.
  • 総ページ数: 145-166
  • 出版者: Springer-Verlag, Heidelberg
• [図書] The role of membrane transport in the detoxification and accumulation of zinc in plants. In A Soil Biology Series: Detoxification of Heavy Metals (2011)
  • 著者名/発表者名: Kawachi, M., Kobae, Y., Tomioka, R., and Maeshima, M.
  • 総ページ数: 129-142
  • 出版者: Springer-Verlag
• [図書] Application of radioisotope in biochemical analysis: Metal binding proteins and metal transporters. In Radioisotopes - Applications in Bio-Medical Science, Ed by Singh, N. (2011)
  • 著者名/発表者名: Kawachi, M., Nagasaki-Takeuchi, N., Kato, M., and Maeshima, M.
  • 総ページ数: 115-126
  • 出版者: InTech
• [備考]
  • URL: http://celld.agr.nagoya-u.ac.jp/index.html