嫌気微生物の脱塩素活性を高める固体電子メディエータの化学構造と活性化機構の解明

2013 年度 研究成果報告書

• 研究課題/領域番号: 23310055
• 研究種目: 基盤研究(B)
• 配分区分: 補助金
• 応募区分: 一般
• 研究分野: 環境技術・環境材料
• 研究機関: 名古屋大学
• 研究代表者: 片山 新太 名古屋大学, エコトピア科学研究所, 教授 (60185808)
• 研究分担者: 鈴木 大典 名古屋大学, エコトピア科学研究所, 助教 (10591076)
• 研究期間 (年度): 2011-04-01 – 2014-03-31
• キーワード: 固体電子メディエータ / 脱ハロゲン反応 / 芳香族ハロゲン化合物 / 酸化還元中心 / 細胞外電子伝達系

研究概要

土壌・底質に分布する腐植物質の中で酸でもアルカリでも不溶の固体腐植ヒューミンが、ペンタクロロフェノール（PCP)を還元的に脱塩素する嫌気性微生物群の細胞外電子伝達物質として機能することを明らかにした。同じ土壌由来の腐植酸では機能しなかった。FT-IR、NMR、ESR、成分分析、サイクリックボルタンメトリ解析から、ヒューミンの酸化還元中心がキノン骨格および鉄錯体であることが推定された。還元型ヒューミンを電子供与体として、PCPを電子受容体として脱ハロゲン呼吸を行う新規なDehalobacter属細菌の存在が推定された。電子伝達はヒューミンと微生物細胞の間で直接行われていると考えられた。

研究成果

• [雑誌論文] Anaerobic 4-chlorophenol mineralization in an enriched culture under iron-reducing conditions (2014)
  • 著者名/発表者名: Zhiling Li, Daisuke Suzuki, Chunfang Zhang, Suyin Yang, Naoko Yoshida, and Arata Katayama
  • 雑誌名: Journal of Bioscience and Bioengineering 巻: (in press)
  • DOI: 10.1016/j.jbiosc.2014.04.007
• [雑誌論文] Use of a glass bead-containing liquid medium for efficient production of a soil-free culture with polychlorinated biphenyl-dechlorination activity (2013)
  • 著者名/発表者名: Daisuke Suzuki, Daisuke Baba, Velayudhan Satheeja Santhi, Robinson David Jebakumar Solomon, and Arata Katayama
  • 雑誌名: World Journal of Microbiology and Biotechnology 巻: 29 ページ: 1461-1471
  • DOI: 10.1007/s11274-013-1310-8
• [雑誌論文] Involvement of Dehalobacter spp. in the anaerobic dechlorination of 2,4,6-trichlorophenol (2013)
  • 著者名/発表者名: Zhiling Li, Daisuke Suzuki, Chunfang Zhang, Naoko Yoshida, Suyin Yang, and Arata Katayama
  • 雑誌名: Journal of Bioscience and Bioengineering 巻: 116(5) ページ: 602-609
  • DOI: 10.1016/j.jbiosc.2013.05.009
• [雑誌論文] A humin-dependent Dehalobacter species is involved in reductive debromination of tetrabromobisphenol A (2013)
  • 著者名/発表者名: Chunfang Zhang, Zhiling Li, Daisuke Suzuki, Lizhen Ye, Naoko Yoshidaaoko, and Arata Katayama
  • 雑誌名: Chemosphere 巻: 92 ページ: 1343-1348
  • DOI: 10.1016/j.chemosphere.2013.05.051
• [雑誌論文] Effects of lactate and sulfate concentration ratios on the anaerobic mineralization of pentachlorophenol (PCP) in a combined PCP-dechlorinating and phenol-degrading culture (2013)
  • 著者名/発表者名: Suyin Yang, Yasushi Inoue, Arata Katayama
  • 雑誌名: Research Journal of Biotechnology 巻: 8(9) ページ: 4-9
• [雑誌論文] Humin as an electron mediator for microbial reductive dehalogenation (2012)
  • 著者名/発表者名: Chunfang Zhang, Arata Katayama
  • 雑誌名: Environmental Science and Technology 巻: 46 ページ: 6575-6583
  • DOI: 10.1021/es3002025
• [雑誌論文] Polyphasic characterization of two microbial consortia with wide dechlorination spectra for chlorophenols (2012)
  • 著者名/発表者名: Chunfang Zhang, Daisuke Suzuki, Zhiling Li, Lizhen Ye, Arata Katayama
  • 雑誌名: Journal of Bioscience and Bioengineering 巻: 114(5) ページ: 512-517
  • DOI: 10.1016/j.jbiosc.2012.05.025
• [雑誌論文] Long-term anaerobic mineralization of pentachlorophenol in a continuous-flow system using only lactate as an external nutrient (2012)
  • 著者名/発表者名: Zhiling Li, Yasushi Inoue, Daisuke Suzuki, Lizhen Ye and Arata Katayama
  • 雑誌名: Environmental Science and Technology 巻: 47 ページ: 1534-1541
  • DOI: 10.1021/es303784f
• [学会発表] Electron Transfer from a Graphite Electrode Assisted by Solid-Phase Electron Mediator (humin) Immobilization Enhances Microbial Reductive Dechlorination of Pentachlorophenol (2013)
  • 著者名/発表者名: Dongdong Zhang, Chunfang Zhang, Zhiling Li, Daisuke Suzuki, and Arata Katayama
  • 学会等名: International Symposium on EcoTopia Science 2013
  • 発表場所: Nagoya University, Nagoya, Japan
  • 年月日: 20131213-15
• [学会発表] Insoluble iron-humate complex as an electron mediator for microbial reductive dechlorination (2013)
  • 著者名/発表者名: Chunfang Zhang, Dongdong Zhang, Zhiling Li, Tetsuji Akatsuka, Suying Yang, Daisuke Suzuki, and Arata Katayama
  • 学会等名: 第29回日本微生物生態学会
  • 発表場所: 鹿児島大学、日本
  • 年月日: 20131123-24
• [学会発表] Towards an Understanding of the Role of Humin as a Solid-phase Electron Mediator for Anaerobic Respiration (2013)
  • 著者名/発表者名: Chunfang Zhang, Lizhen Ye, Toyoko Demachi, Daisuke Suzuki, and Arata Katayama
  • 学会等名: the 21st International Symposium on Environmental Biogeochemistry (ISEM2013)
  • 発表場所: Wuhan, China
  • 年月日: 20131013-18
• [学会発表] Anaerobic organohalide degradation by multiple microbial species for bioremediation (2013)
  • 著者名/発表者名: Arata Katayama
  • 学会等名: Launching Ceremony of IWA China Anaerobic Digestion Committee & Sino-Dutch AD Technology Workshop
  • 発表場所: Harbin Institute of Technology, Harbin, China
  • 年月日: 2013-05-06
  • 招待講演
• [学会発表] Microbial cofinment technology of groundwater contaminated with halogenated organic pollutants (2012)
  • 著者名/発表者名: Arata Katayama, Zhiling Li., Chunfang Zhang, Dongdong Zhang, Suyin Yang, and Daisuke Suzuki
  • 学会等名: First International Symposium on Advanced Water Science and Technology (ISAWST-1)
  • 発表場所: Noyori Conference Hall, Nagoya University, Nagoya, Japan
  • 年月日: 20121111-13
  • 招待講演
• [学会発表] Microbial reductive dechlorination of pentachlorophenol mediated by humin (poster) (2012)
  • 著者名/発表者名: Chunfang Zhang, Zhiling Li., Daisuke Suzuki, and Arata Katayama
  • 学会等名: First International Symposium on Advanced Water Science and Technology (ISAWST-1)
  • 発表場所: Noyori Conference Hall, Nagoya University, Nagoya, Japan
  • 年月日: 20121111-13
• [学会発表] Spatial distribution of functional populatioins in a long-term maintained column for pentachlorophenol (PCP) anaerobic mineralization (poster) (2012)
  • 著者名/発表者名: Zhiling Li., Daisuke Suzuki, Chunfang Zhang, Yasushi Inoue, and Arata Katayama
  • 学会等名: First International Symposium on Advanced Water Science and Technology (ISAWST-1)
  • 発表場所: Noyori Conference Hall, Nagoya University, Nagoya, Japan
  • 年月日: 20121111-13
• [学会発表] Influence of electron donors and electron acceptors on the 3-chlorophenol dechlorination activity of anaerobic enrichment culture (poster) (2012)
  • 著者名/発表者名: Daisuke Suzuki, Zhiling Li., Chunfang Zhang, and Arata Katayama
  • 学会等名: First International Symposium on Advanced Water Science and Technology (ISAWST-1)
  • 発表場所: Noyori Conference Hall, Nagoya University, Nagoya, Japan
  • 年月日: 20121111-13
• [学会発表] Long.term Anaerobic Mineralization of PCP in a Continuous.Flow System Using Only Lactate as an External Nutrient (Oral) (2012)
  • 著者名/発表者名: Zhiling Li, Yasushi Inoue, Daisuke Suzuki, Lizhen Ye and Arata Katayama
  • 学会等名: the 4th International Conference on Soil Pollution and Remediation (SOILREM 2012)
  • 発表場所: Yantai, China
  • 年月日: 20120923-26
• [学会発表] A novel Dehalobacter sp. is involved in anaerobic dechlorination of Trichlorophenol (TCP) (Oral) (2012)
  • 著者名/発表者名: Zhiling Li, Daisuke Suzuki, Chunfang Zhang, Arata Katayama
  • 学会等名: The 15th International biotechnology Synposium and Exhibition (IBS)
  • 発表場所: Daegu, Korea
  • 年月日: 20120916-21
• [学会発表] Characterization of a Dehalobacter-dominating consortium that debrominates Tetrabromobisphenol A (poster) (2012)
  • 著者名/発表者名: Chunfang Zhang, Zhiling Li, Daisuke Suzuki, Lizhen Ye, Naoko Yoshida and Arata Katayama
  • 学会等名: The power of the small-the 14th International Symposium on Microbial Ecology .ISME-14
  • 発表場所: The Bella Center , Copenhagen Denmark
  • 年月日: 20120819-24
• [学会発表] Characteristics of a 4-chlorophenol degrading, sulfate-reducing bacterium isolated from a river sediment contaminated with chlorinated solvents (poster) (2012)
  • 著者名/発表者名: Daisuke Suzuki, Arata Katayama
  • 学会等名: The power of the small-the 14th International Symposium on Microbial Ecology .ISME-14
  • 発表場所: The Bella Center , Copenhagen Denmark
  • 年月日: 20120819-24
• [学会発表] Polyphasic characterization of two anaerobic microbial consortia dechlorinating wide spectra of phenols with lower levels of chlorination (2012)
  • 著者名/発表者名: Chunfang Zhang, Daisuke Suzuki, Zhiling Li, Lizhen Ye, and Arata Katayama
  • 学会等名: WET2012 Water and Environment Technology Conference
  • 発表場所: The University of Tokyo, Tokyo, Japan
  • 年月日: 20120628-30
• [学会発表] Enrichment of 4-chlorophenol-degrading culture under both iron-reducing and sulfate-reducing conditions (Oral) (2011)
  • 著者名/発表者名: Zhiling Li, Suyin Yang, Daisuke Suzuki, and Arata Katayama
  • 学会等名: International Symposium on EcoTopia Science 2011
  • 発表場所: Nagoya University, Nagoya, Japan
  • 年月日: 20111209-11
• [学会発表] Simulation of the reductive dechlorination processes in a lab-scale anaerobic biobarrier with the enriched TCP dechlorination consortium (Oral) (2011)
  • 著者名/発表者名: Zhiling Li, Yasushi Inoue, Takuya Mizoguchi, Yohei Simizu, Naoko Yoshida and Arata Katayama
  • 学会等名: The International Conference on 7th Environmental Anaerobic Technologies and Bioenergy
  • 発表場所: Tianjin University, Tianjin, China
  • 年月日: 20111112-13
• [学会発表] Characterization of microbial community dechlorinating PCP to phenol with the presence of soil (Oral) (2011)
  • 著者名/発表者名: Chunfang Zhang, Lizhen Ye, Zhiling Li, Daisuke Suzuki, Toyoko Demachi, and Arata Katayama
  • 学会等名: The 4th IWA-ASPIRE Tokyo
  • 発表場所: Japan
  • 年月日: 20111002-06
• [学会発表] Microbial reductive debromination of Tetrabromobisphenol A (Oral) (2011)
  • 著者名/発表者名: Chunfang Zhang, Zhiling Li, Daisuke Suzuki, and Arata Katayama
  • 学会等名: 第14回水環境学会シンポジウム
  • 発表場所: 仙台
  • 年月日: 20110910-11
• [学会発表] The microbial spatial distribution in a long-term maintained biobarrier for pentachlorophenol (PCP) anaerobic mineralization (Oral) (2011)
  • 著者名/発表者名: Zhiling Li, Yasushi Inoue, Daisuke Suzuki, Arata Katayama
  • 学会等名: 第14回水環境学会シンポジウム
  • 発表場所: 仙台
  • 年月日: 20110910-11
• [学会発表] Anaerobic degradation of aromatic compounds by microorganisms in paddy field (2011)
  • 著者名/発表者名: Arata Katayama, Naoko Yoshida, Atsuchi Shibata, Daisuke Baba, Suyin Yang, Zhiling Li, Hyou-Ken Kim, Chunfang Zhang, and Daisuke Suzuki
  • 学会等名: KSEA's 30th Anniversary International Symposium on Environment and Food Safety for the Future Generation
  • 発表場所: the international Convention Center Jeju , Korea
  • 年月日: 20110707-09
  • 招待講演
• [産業財産権] 電子伝達システムおよびその用途 (2013)
  • 発明者名: 片山新太、章春芳、張冬冬、李智灵、鈴木大典
  • 権利者名: 名古屋大学
  • 産業財産権種類: 特願
  • 産業財産権番号: 2013-158394
  • 出願年月日: 2013-07-31