紀伊半島のＡＬＳとパーキンソン認知症複合患者由来ｉＰＳ細胞の作成と神経細胞誘導

2013 年度 研究成果報告書

• 研究課題/領域番号: 23591242
• 研究種目: 基盤研究(C)
• 配分区分: 基金
• 応募区分: 一般
• 研究分野: 神経内科学
• 研究機関: 三重大学
• 研究代表者: 小久保 康昌 三重大学, 地域イノベーション学研究科, 招へい教授 (60263000)
• 研究分担者: 葛原 茂樹 鈴鹿医療科学大学, 保健衛生学部, 教授 (70111383) ; 広川 佳史 三重大学, 大学院医学系研究科, 講師 (30322738)
• 連携研究者: 江良 拓実 熊本大学, 発生医学研究所, 教授 (00273706)
• 研究期間 (年度): 2011 – 2013
• キーワード: 紀伊半島 / ALS/PDC / iPS細胞 / 神経変性疾患

研究概要

Kii ALS/PDC患者５名の血液検体を用いて、疾患特異的iPS細胞を樹立した。各症例間でT細胞やiPSCの樹立効率・増殖速度は異なっていた。今後、神経系解析に用いるiPSCクローンの選抜を進め、各種神経細胞やグリア細胞といった神経系細胞への分化誘導を行い、病態の解明や新たな治療に迫る検討を行っていく。

研究成果

• [雑誌論文] Cerebrospinal Fluid Biomarkers for Kii Amyotrophic Lateral Sclerosis /Parkinsonism-Dementia Complex (2013)
  • 著者名/発表者名: Yui Nakayama, Satoru Morimoto, Misao Yoneda, Shigeki Kuzuhara, and Yasumasa Kokubo
  • 雑誌名: Journal of Neurodegenerative Diseases 巻: Article ID 679089 ページ: 4
  • 査読あり
• [雑誌論文] Analyses of the MAPT, PGRN, and C9orf72 mutations in Japanese patients with FTLD, PSP, and CBS (2013)
  • 著者名/発表者名: Kotaro Ogaki,Yuanzhe Li,Masashi Takanashi,Kei-Ichi Ishikawa,Tomonori Kobayashi,Takashi Nonaka,Masato Hasegawa, Masahiko Kishi,Hiroyo Yoshino,Manabu Funayama,Tetsuro Tsukamoto,Keiichi Shioya, Masayuki Yokochi,Hisamasa Imai,Ryogen Sasaki,Yasumasa Kokubo,Shigeki Kuzuhara,Hiroyuki Tomiyama,,Nobutaka Hattori
  • 雑誌名: Parkinsonism Relat Disord 巻: 19(1) ページ: 15-20
  • 査読あり
• [雑誌論文] Environmental Characteristics and oidative stress of inhabitants and patinets with amyoyrophic lateral sclerosis in a high-incidence area on the Kii peninsula, Japan (2013)
  • 著者名/発表者名: Tameko Kihira, Kazushi Okamoto, Sohei Yoshida, Tetuya Kondo, Keiko Iwai, Sachiko Wada, Yoshinori Kajimoto, Tomoyoshi Kondo, Yasumasa Kokubo, Shigeki Kuzuhara
  • 雑誌名: Internal Medicine 巻: 52 ページ: 1479-1486
  • 査読あり
• [雑誌論文] Rare case of disseminated cysticercosis and taeniasis in a Japanese traveller after returning from India (2013)
  • 著者名/発表者名: Ken-ichiro Kobayashi, Fukumi Nakamura-Uchiyama, Takeshi Nishiguchi, Kenichi Isoda, Yasumasa Kokubo, Katsuhiko Ando, Masaki Katurahara, Yasuhito Sako, Tetsuya Yanagida, Akira Ito, Sentaro Iwabuchi, and Kenji Ohnishi
  • 雑誌名: Am J Trop Med Hyg 巻: 89(1) ページ: 58-62
  • 査読あり
• [雑誌論文] α-Synuclein Pathology in Amyotrophic Lateral Sclerosis/Parkinsonism Dementia Complex in the Kii Peninsula, Japan (2012)
  • 著者名/発表者名: Kokubo Y, Taniguchi A, Hasegawa M, Hayakawa Y, Morimoto S, Yoneda M, Hirokawa Y, Shiraishi T, Saito Y, Murayama S, Kuzuhara S
  • 雑誌名: J Neuropathol Exp Neurol 巻: 71 ページ: 625-630
  • 査読あり
• [雑誌論文] Cardiac (123)I-meta-iodobenzylguanidine scintigraphy in patients with amyotrophic lateral sclerosis and parkinsonism-dementia complex of the Kii peninsula, Japan (2012)
  • 著者名/発表者名: Kokubo Y, Nomura Y, Morimoto S, Kuzuhara S
  • 雑誌名: Parkinsonism and Related Disorders 巻: 18 ページ: 306-308
  • 査読あり
• [雑誌論文] Mutational analysis of familial and sporadic amyotrophic lateral sclerosis with OPTN mutations in Japanese population (2012)
  • 著者名/発表者名: Naruse H, Takahashi Y, Kihira T, Yoshid S, Kokubo Y, Kuzuhara S, Ishiura H, Amagasa M, Murayama S, Tsuji S, Goto J
  • 雑誌名: Amyotroph Lateral Scler 巻: 13 ページ: 562-566
  • 査読あり
• [雑誌論文] An increase in ALS incidence on the Kii Peninsula, 1960-2009: A possible link to change in drinking water source (2012)
  • 著者名/発表者名: Tameko Kihira, S Yoshida, T Kondo, K Iwai, S Wada, S Morinaga, Y Kazimoto, T Kondo, K Okamoto, Y Kokubo, S Kuzuhara
  • 雑誌名: Amyotrophic Lateral Sclerosis 巻: 13 ページ: 347-350
  • 査読あり
• [雑誌論文] Amyotrophic Lateral Sclerosis/Parkinsonism Dementia Complex in the Kii Peninsula of Japan (Muro disease): a review w on recent research and new concept (2012)
  • 著者名/発表者名: Kuzuhara S, Kokubo Y
  • 雑誌名: Amyotrophic Lateral Sclerosis and the frontotemporal dementias(Oxford University Press, Ed. By Michael J. Strong) ページ: 39-54
  • 査読あり
• [雑誌論文] α-Synuclein pathology in the amyotrophic lateral sclerosis/parkinsonism dementia complex in the Kii Peninsula, Japan (2012)
  • 著者名/発表者名: Kokubo Y, Hirokawa Y, Shiraishi T, Kuzuhara S.ほか
  • 雑誌名: J Neuropathol Exp Neurol 巻: 71(7) ページ: 625-630
  • 査読あり
• [雑誌論文] Cardiac 123I-Meta- Iodobenzylguanidine Scintigraphy and Lewy Body Pathology in a Patient with Amyotrophic Lateral Sclerosis and Parkinsonism–Dementia Complex of Kii, Japan (2011)
  • 著者名/発表者名: Kokubo Y, Morimoto S, Shindo A, Hirokawa Y, Shiraishi T, Saito Y, Murayama S, Kuzuhara S
  • 雑誌名: Mov Disord 巻: 26 ページ: 401-403
  • 査読あり
• [雑誌論文] PLA2G6 variant in Parkinson's disease (2011)
  • 著者名/発表者名: Hiroyuki Tomiyama, Hiroyo Yoshino, Kotaro Ogaki, Lin Li, Chikara Yamashita, Yuanzhe Li, Manabu Funayama, Ryogen Sasaki, Yasumasa Kokubo, Shigeki Kuzuhara, and Nobutaka Hattori
  • 雑誌名: J Hum Genet 巻: 56(5) ページ: 401-403
  • 査読あり
• [学会発表] Clinical trial of Edaravone in amyotrophic lateral sclerosis/parkinsonism-dementia complex of the Kii peninsula of Japan (2013)
  • 著者名/発表者名: Y. Kokubo, S. Morimoto, T. Nakagawa, K. Miyazaki, S. Kuzuhara
  • 学会等名: 24th International Symposium on ALS/MND
  • 発表場所: Milan,Italy
  • 年月日: 20131206-08
• [学会発表] Transitional metal contents in scalp hair and lifestyle of ALS patients and residents in the Kii Peninsula, Japan -the second report- (2013)
  • 著者名/発表者名: Tameko Kihira, Iori Sakurai, Sohei Yoshida, Ikuro Wakayama, Koichi Takamiya, Yukihiro Nakano, Ryo Okumura, Iinuma Yuhto, Keiko Iwai, Junko Kohmoto, Yoshinori Kajimoto, Yasuhiro Hiwatani, Kazushi Okamoto, Yasumasa Kokubo, Shigeki Kuzuhara
  • 学会等名: 24th International Symposium on ALS/MND
  • 発表場所: Milan,Italy
  • 年月日: 20131206-08
• [学会発表] Immunohistochemical study of the cerebellum in amyotrophic lateral screlosis (ALS)/ parkinson-dementia complex (PDC) in Kii peninsula (ALS/PDC- Kii, Muro disease) (2013)
  • 著者名/発表者名: Satoru Morimoto, Yasumasa Kokubo , Hiroyuki Hatsuta, Shigeki Kuzuhara, Shigeo Murayama
  • 学会等名: Alzheimer's Association International Conference
  • 発表場所: Boston, USA
  • 年月日: 20130713-17
• [学会発表] Amyotrophic lateral sclerosis (ALS) and parkinsonism-dementia complex (PDC) of the Kii peninsula of Japan (Muro disease). - Recent Advances of Reserch - (2013)
  • 著者名/発表者名: Yasumasa Kokubo
  • 学会等名: 9th international congress on mental disorders & other non-motor features in Parkinson's diseaseand related disorders. MDPD 2013. in Seoul
  • 発表場所: South Korea
  • 年月日: 20130418-21
• [学会発表] Alfa-Synuclein pathology of amyotrophic lateral sclerosis/parkinsonism-dementia complex in the Kii Peninsula, Japan (2012)
  • 著者名/発表者名: Yasumasa Kokubo, Akira Taniguchi, Masato Hasegawa, Yuma Hayakawa, Satoru Morimoto, Misao Yoneda, Yoshihumi Hirokawa, Taizo Shiraishi, Yuko Saito, Shigeo Murayama, Shigeki Kuzuhara
  • 学会等名: The 8^<th> International Conference on Frontotemporal dementias
  • 発表場所: Manchester, UK
  • 年月日: 20120905-07
• [学会発表] Immunochemical Study Western blotting of tauopathy in ALS/ Parkinson Dementia Complex (PDC), Kii ,Japan (2012)
  • 著者名/発表者名: Satoru Morimoto,Yasumasa Kokubo, Masato Hasegawa, Shigeki Kuzuhara,Shigeo Murayama
  • 学会等名: AANP
  • 発表場所: Chicago, USA
  • 年月日: 20120621-24
• [学会発表] Changing patterns of amyotrophic lateral sclerosis (ALS) and parkinsonism-dementia complex (PDC) of the Kii peninsula in Japan (2012)
  • 著者名/発表者名: Shigeki Kuzuhara、Yasumasa Kokubo
  • 学会等名: 13th Asian Oceanian Congress of Neurology
  • 発表場所: Melbourne, Australia
  • 年月日: 20120604-08
• [学会発表] NEUROPSYCHOLOGICAL STUDY OF PATIENTS WITH AMYOTROPHIC LATERAL SCLEROSIS/PARKINSONISM-DEMENTIA COMPLEX OF THE KII PENINSULA OF JAPAN (2011)
  • 著者名/発表者名: SHINDO A,KUZUHARA S,KOKUBO Y
  • 学会等名: 22th International Symposium on ALS/MND
  • 発表場所: Sydoney,Australia
  • 年月日: 20111130-1202
• [図書] Amyotrophic lateral sclerosis-parkinsonism-dementia complex in the Kii peninsula of Japan(Muro disease):a review on recent research and new concept (2012)
  • 著者名/発表者名: Shigeki Kuzuhara, Yasumasa Kokubo.et.
  • 総ページ数: 39-54
  • 出版者: Oxford University Press
• [備考] 紀伊半島の筋萎縮性総索硬化症/パーキンソン認知症複合-Kii ALS/PDC-
  • URL: http://kii-als-pdc-project.com