研究課題
Determined the biotransformation capabilities of Sphingobium bacterium when it was exposed to different polyalkyl chain-alkylated monoaromatic and polycyclic aromatic hydrocarbons (PAHs) and constructed biotransformation pathways for their biodegradation by comprehensive liquid chromatography electrospray ionization tandem mass spectrometry.Cooperative biodegradation of a model asphaltene long-chain PAH was investigated by a compartmentalized approach using bacteria belonging to the genera Pseudomonas and Sphingobium. Pseudomonas reduced the size (and molar mass) of 1-dodecylnaphthalene through subterminal alkyl chain cutting resulting in shorter alkyl chain polyaromatics that were further biotransformed by Sphingobium to ultimately produce aromatic ring-opened chain-shortened compounds.
2: おおむね順調に進展している
Determined mechanisms of biotransformation of chain-alkylated PAHs by Sphingobium. Made progress on the application of high resolution mass spectrometry (HR-MS) methods development for analyzing cooperative bacterial biotransformation of a model asphaltene compound.
Develop methods for structural predictions and docking simulations of Sphingobium and Pseudomonas oxygenase enzymes and target compounds. Conduct biodegradation experiments using heavy oil. Produce heavy oil fractions from heavy oil to be used in bacterial biotransformation assays. Develop methods for IR analyses to evaluate biotransformation of complex hydrocarbon mixtures. Investigate the biotransformation of tethered aromatic compounds.
Planning to purchase second-hand equipment, therefore saved the budget from 2023 for this purpose.
すべて 2024 2023
すべて 雑誌論文 (4件) (うち国際共著 4件、 査読あり 4件、 オープンアクセス 2件) 学会発表 (7件) (うち国際学会 2件)
Biodegradation
巻: 34 ページ: 215～233
10.1007/s10532-023-10014-9
Marine Genomics
巻: 72 ページ: 101070～101070
10.1016/j.margen.2023.101070
Microbiology Resource Announcements
巻: 12 ページ: e00599-23
10.1128/mra.00599-23
Microbiology Spectrum
巻: 11 ページ: e04837-22
10.1128/spectrum.04837-22
