| 研究課題/領域番号 |
23K05713
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| 研究機関 | 金沢大学 |
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研究代表者 |
NGO XUANKIEN 金沢大学, ナノ生命科学研究所, 特任助教 (60778190)
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| 研究期間 (年度) |
2023-04-01 – 2026-03-31
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| キーワード | ABC transporter / MsbA / LptC / HS-AFM / E. coli / FRET |
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| 研究実績の概要 |
1)Successfully prepared 2D crystal structures of MsbA within lipid membranes containing both with and without Kdo2 lipid A; 2) Achieved high spatial and temporal imaging of both NBD and TMD sides of MsbA embedded in these lipid membranes; 3) Developed new methods for the automatic analysis of AFM data; 4) completed the construction of plasmids and transfected E. coli cells with the target plasmids aimed for FRET measurements.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
1: 当初の計画以上に進展している
理由
I am potentially investigating a novel pathway for the gate operation of Lipopolysaccharide (LPS) induced by ABC transporters MsbA and LptC in E. coli.
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| 今後の研究の推進方策 |
In FY2024, my planned research agenda includes the following tasks: 1)Finalize the data analyses of AFM data; 2) Develop a protein-membrane model suitable for Molecular Dynamics simulation and/or Coarse-Grained simulation; 3) Investigate the interaction of MsbA with target protein in E. coli using FRET techniques; 4) Draft the manuscript detailing the findings.
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| 備考 |
I am a co-chairman organizing The First International Symposium of Nano Life Science: NanoBioCoM2023.
https://www.icisequynhon.com/conferences/2023/nanobiocom2023/index.html
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