| 研究実績の概要 |
The purpose of our research is to visualize deep living tissue through fluorescence imaging because fluorescence guided cancer surgery is very important for complete removal of solid tumors. So far visible and near-infrared (NIR) region as been used for clinical applications. For deep tissue visualization, shortwave-infrared (SWIR, >1000 nm) emitting fluorophores are important. I proposed to synthesize water soluble, biocompatible, and SWIR emitting organic fluorophores for deep tissue imaging of breast cancer. The intermediate compounds to synthesize proposed organic probe was unstable so, we alternatively developed organic probes which are highly efficient for the proposed biological applications.
We demonstrate that these probes are biocompatible and versatile, which can be conjugated to any antibodies and proteins. Using newly synthesized probes, we carried out in-vivo imaging of breast cancer. Also we demonstrate that these probes can be applicable for multicolor imaging of cancers and blood vessels. Currently, we are working on the development of clinically applicable probes with bright fluorescence for breast cancer imaging. The results obtained from this work is recently published in ACS Applied Materials and Interfaces 2024, 16, 14, 17253-17266.
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| 今後の研究の推進方策 |
Some of the intermediate compounds needed to synthesize the proposed organic probe was very low so, we could synthesized alternate probes which are efficient for fluorescence imaging in mice experiments. The water solubility of the developed probes are relatively low, it limits these probes from the clinical application in human. In future we will be carrying out experiments to enhance their water solubility and perform cancer imaging animal model.
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| 次年度使用額が生じた理由 |
In the fiscal year 2023, the grant amount was mostly used for chemicals and research collaboration visit. In the fiscal year 2024, we are planning to carry out more animal experiments which required expensive protein and antibody molecules and a part of the grant amount will be spent for collaboration visit and to attend international conferences. the incurring amount will be properly used in the fiscal year 2024.
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