| 研究課題/領域番号 |
23K13862
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| 研究機関 | 東北大学 |
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研究代表者 |
ELLEN 東北大学, 工学研究科, 特任助教 (40870176)
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| 研究期間 (年度) |
2023-04-01 – 2025-03-31
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| キーワード | K+ transporter / membrane protein / potassium ion |
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| 研究実績の概要 |
K+ is an essential cation that has many important functions for living cells. Cells tend to accumulate high intracellular concentrations of K+ via protein transporters located in the cell membrane. The Trk/Ktr/HKT K+ transporter family is one of the main K+ transporters found in non-animal cells, including bacteria, yeast,and plants. In the model organism, Escherichia coli K-12, Trk transporter is further divided into two types: TrkG and TrkH. Although they are homologous protein, previously, we had found the differences in their uptake activities. But the information about their gene regulation is very limited. In this study, we investigate the expression of trkG and trkH in relation to various factors, such as salt stress, extracellular K+ concentration and xenogenic silencing proteins.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
Factors affecting gene expression regulation of trkG and TrkH in Escherichia coli K-12 were examined. Since TrkG activity is closely linked to Na+, the impact of salt (NaCl) stress on its expression was investigated. Interestingly, trkG expression remained unaffected by NaCl stress. In addition, the expression of trkG and trkH was also independent of K+ concentrations in the external enviornment, suggesting they most likely to be expressed consitutively. The most notable differences between trkG and trkH expression is in the involvment of the xenogenic silencing proteins. trkG expression significantly increased in their absence (using knock-out mutant) compared to unchanged trkH. However, trkG expression persists in the wild-type, suggesting incomplete silencing of trkG by those proteins.
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| 今後の研究の推進方策 |
In our forthcoming research, I aim to elucidate the role of TrkG in the K+ uptake system of E. coli K-12. Despite E. coli's efficiency, the retention of both Trk transporters remains puzzling. Utilizing knock-out mutants, I will assess various stress conditions to pinpoint when trkG activity becomes vital for E. coli growth. Additionally, I plan to employ my own E. coli K+ transporter deficient mutants to identify functional K+ transporters in the plant model organism, Arabidopsis thaliana. Potassium is crucial for growth, reproduction, and productivity, and the discovery of new plant K+ transporters using our library of A. thaliana membrane proteins will significantly enhance our understanding of plant nutrient uptake and stress responses.
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| 次年度使用額が生じた理由 |
I could save up some reagents and article costs. I will the budget next fiscal year for going to international conference to present my result
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