| 研究課題/領域番号 |
23K13898
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| 研究機関 | 国立研究開発法人理化学研究所 |
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研究代表者 |
Vo NgocQuynhNhu 国立研究開発法人理化学研究所, 環境資源科学研究センター, 特別研究員 (60888543)
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| 研究期間 (年度) |
2023-04-01 – 2026-03-31
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| キーワード | Terpene synthases / Drimenol / Sesquiterpenes / Marine bacteria / Gene clusters / Blue-light switch / Metabolic engineering |
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| 研究実績の概要 |
The goal of this study is to understand the diversity and biosynthetic potential of terpenoids in bacteria, especially marine bacteria. To achieve this goal, during the FY2023, as originally planned, I have investigated and found a number of promising bacterial terpene synthase genes, using our optimized genomic data mining method. I have characterized several terpene synthase enzymes by in vitro enzymatic assays and gas chromatography-mass spectrometry, and discovered novel enzymatic activities.
Moreover, I have successfully found the biosynthetic genes clustered with drimenol synthases encoded in marine bacterial genomes for drimenol synthesis, which are uniquely conserved. I have also verified the drimenol production in marine bacteria and suggested the evolutionary features of the drimenol gene cluster in marine bacteria. We have summarized these findings in a scientific paper and we will submit it for publication.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
2: おおむね順調に進展している
理由
As stated in the "Summary of Research Achievements", I have investigated and found more unknown terpene synthase genes of bacterial origin by genomic data mining and biochemical validation. In addition, I have elucidated the existence of core biosynthetic genes involved in drimenol biosynthesis and their distribution across marine bacterial genomes, verifying the accumulation of drimenol, a highly active sesquiterpene, in bacteria (manuscript in preparation). With those achievements, the status to date of my research project is progressing smoothly, which is approaching to the goal of advancing our understanding of the diversity and biosynthetic origin of terpenoids derived from bacteria including marine bacteria.
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| 今後の研究の推進方策 |
As for future work, I will further perform functional analysis using in vitro enzymatic assays and gas chromatography-mass spectrometry of promising candidate terpene synthases harvested from data mining approach to identify more novel enzymes with unique structures and reaction mechanisms that can make novel active terpenoids. In addition, I will elucidate biosynthetic genes, with a focus on prenyltransferases and monooxygenases, clustered with those characterized terpene synthases to make structurally divergent compounds. At the same time, I will construct and characterize the new bacterial blue-light switch platform to be ready for the introduction of novel bacterial terpene synthase genes and their clustered genes to enhance the production of target compounds.
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| 次年度使用額が生じた理由 |
During FY2023, I have applied new genomic data mining strategies that led me to harvest more unknown promising terpene synthase genes hidden in the bacterial genomes. I have successfully identified several novel enzymatic activities, so I have characterized those new enzymes in detail and prepared for the manuscript for publication. Therefore, I have decided to analyze the functions of other candidate genes and construct the blue-light switch system in the next FY2024. For these reasons, I have the remaining budget to be used for the next fiscal year. I plan to combine the "Amount to be used next fiscal year" with the grant requested for the FY2024 to purchase reagents and synthetic genes for characterizing other candidate genes in detail and performing blue-light experiments.
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